دورية أكاديمية

KLF4 regulates skeletal muscle development and regeneration by directly targeting P57 and Myomixer

التفاصيل البيبلوغرافية
العنوان: KLF4 regulates skeletal muscle development and regeneration by directly targeting P57 and Myomixer
المؤلفون: Shufang Cai, Xiaoyu Wang, Rong Xu, Ziyun Liang, Qi Zhu, Meilin Chen, Zhuhu Lin, Chenggan Li, Tianqi Duo, Xian Tong, Enru Li, Zuyong He, Xiaohong Liu, Yaosheng Chen, Delin Mo
المصدر: Cell Death and Disease, Vol 14, Iss 9, Pp 1-14 (2023)
بيانات النشر: Nature Publishing Group, 2023.
سنة النشر: 2023
المجموعة: LCC:Cytology
مصطلحات موضوعية: Cytology, QH573-671
الوصف: Abstract Krüppel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription factor that regulates diverse cellular processes such as cell proliferation, apoptosis, and differentiation. Our previous study showed that KLF4 expression is upregulated in skeletal muscle ontogeny during embryonic development in pigs, suggesting its importance for skeletal muscle development and muscle function. We revealed here that KLF4 plays a critical role in skeletal muscle development and regeneration. Specific knockout of KLF4 in skeletal muscle impaired muscle formation further affecting physical activity and also defected skeletal muscle regeneration. In vitro, KLF4 was highly expressed in proliferating myoblasts and early differentiated cells. KLF4 knockdown promoted myoblast proliferation and inhibited myoblast fusion, while its overexpression showed opposite results. Mechanically, in proliferating myoblasts, KLF4 inhibits myoblast proliferation through regulating cell cycle arrest protein P57 by directly targeting its promoter; while in differentiated myoblasts, KLF4 promotes myoblast fusion by transcriptionally activating Myomixer. Our study provides mechanistic information for skeletal muscle development, reduced muscle strength and impaired regeneration after injury and unveiling the mechanism of KLF4 in myogenic regulation.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2041-4889
Relation: https://doaj.org/toc/2041-4889
DOI: 10.1038/s41419-023-06136-w
URL الوصول: https://doaj.org/article/ea73aa74ce0649f9a9514796d5f87d01
رقم الأكسشن: edsdoj.73aa74ce0649f9a9514796d5f87d01
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20414889
DOI:10.1038/s41419-023-06136-w