دورية أكاديمية
In-depth Analysis of the Lid Subunits Assembly Mechanism in Mammals
العنوان: | In-depth Analysis of the Lid Subunits Assembly Mechanism in Mammals |
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المؤلفون: | Minghui Bai, Xian Zhao, Kazutaka Sahara, Yuki Ohte, Yuko Hirano, Takeumi Kaneko, Hideki Yashiroda, Shigeo Murata |
المصدر: | Biomolecules, Vol 9, Iss 6, p 213 (2019) |
بيانات النشر: | MDPI AG, 2019. |
سنة النشر: | 2019 |
المجموعة: | LCC:Microbiology |
مصطلحات موضوعية: | 26S proteasome, 19S regulatory particle, lid subcomplex, Rpn proteins, assembly, Microbiology, QR1-502 |
الوصف: | The 26S proteasome is a key player in the degradation of ubiquitinated proteins, comprising a 20S core particle (CP) and a 19S regulatory particle (RP). The RP is further divided into base and lid subcomplexes, which are assembled independently from each other. We have previously demonstrated the assembly pathway of the CP and the base by observing assembly intermediates resulting from knockdowns of each proteasome subunit and the assembly chaperones. In this study, we examine the assembly pathway of the mammalian lid, which remains to be elucidated. We show that the lid assembly pathway is conserved between humans and yeast. The final step is the incorporation of Rpn12 into the assembly intermediate consisting of two modular complexes, Rpn3-7-15 and Rpn5-6-8-9-11, in both humans and yeast. Furthermore, we dissect the assembly pathways of the two modular complexes by the knockdown of each lid subunit. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2218-273X |
Relation: | https://www.mdpi.com/2218-273X/9/6/213; https://doaj.org/toc/2218-273X |
DOI: | 10.3390/biom9060213 |
URL الوصول: | https://doaj.org/article/c73afcab3f654cb0a8be15c586f8faf6 |
رقم الأكسشن: | edsdoj.73afcab3f654cb0a8be15c586f8faf6 |
قاعدة البيانات: | Directory of Open Access Journals |
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