دورية أكاديمية
Enzymatic Decontamination of G-Type, V-Type and Novichok Nerve Agents
| العنوان: | Enzymatic Decontamination of G-Type, V-Type and Novichok Nerve Agents |
|---|---|
| المؤلفون: | Pauline Jacquet, Benjamin Rémy, Rowdy P. T. Bross, Marco van Grol, Floriane Gaucher, Eric Chabrière, Martijn C. de Koning, David Daudé |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 15, p 8152 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | decontamination, phosphotriesterase, nerve agents, organophosphorus, Novichok, sarin, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Organophosphorus nerve agents (OPNAs) are highly toxic compounds inhibiting cholinergic enzymes in the central and autonomic nervous systems and neuromuscular junctions, causing severe intoxications in humans. Medical countermeasures and efficient decontamination solutions are needed to counteract the toxicity of a wide spectrum of harmful OPNAs including G, V and Novichok agents. Here, we describe the use of engineered OPNA-degrading enzymes for the degradation of various toxic agents including insecticides, a series of OPNA surrogates, as well as real chemical warfare agents (cyclosarin, sarin, soman, tabun, VX, A230, A232, A234). We demonstrate that only two enzymes can degrade most of these molecules at high concentrations (25 mM) in less than 5 min. Using surface assays adapted from NATO AEP-65 guidelines, we further show that enzyme-based solutions can decontaminate 97.6% and 99.4% of 10 g∙m−2 of soman- and VX-contaminated surfaces, respectively. Finally, we demonstrate that these enzymes can degrade ethyl-paraoxon down to sub-inhibitory concentrations of acetylcholinesterase, confirming their efficacy from high to micromolar doses. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/22/15/8152; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms22158152 |
| URL الوصول: | https://doaj.org/article/7619fbb7f09f4a11a9da56d0bcaf4409 |
| رقم الأكسشن: | edsdoj.7619fbb7f09f4a11a9da56d0bcaf4409 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms22158152 |