دورية أكاديمية
Regulated expression of miR-155 is required for iNKT cell development
العنوان: | Regulated expression of miR-155 is required for iNKT cell development |
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المؤلفون: | Alessia eBurocchi, Paola ePittoni, Esmerina eTili, Alice eRigoni, Stefan eCostinean, Carlo Maria Croce, MARIO Paolo COLOMBO |
المصدر: | Frontiers in Immunology, Vol 6 (2015) |
بيانات النشر: | Frontiers Media S.A., 2015. |
سنة النشر: | 2015 |
المجموعة: | LCC:Immunologic diseases. Allergy |
مصطلحات موضوعية: | Gene Expression Regulation, microRNA, transgenic, thymic development, iNKT cell, Immunologic diseases. Allergy, RC581-607 |
الوصف: | Invariant Natural Killer T cells (iNKT cells) are CD1d-restricted, lipid antigen–reactive T lymphocytes with immunoregulatory functions. iNKT cell development in the thymus proceeds through subsequent stages, defined by the expression of CD44 and NK1.1, and is dictated by a unique gene expression program, including microRNAs. Here, we investigated whether miR-155, a microRNA involved in differentiation of most hematopoietic cells, played any role in iNKT cell development. To this end, we assessed the expression of miR-155 along iNKT cell maturation in the thymus, and studied the effects of miR-155 on iNKT cell development using Lck-miR-155 transgenic mice, that over express miR-155 in T cell lineage under the lymphocyte-specific protein tyrosine kinase (Lck) promoter. We show that miR-155 is expressed by newly selected immature wild type iNKT cells and turned off along iNKT cells differentiation. In transgenic mice, miR-155 over-expression resulted in a substantial block of iNKT cell maturation at Stage 2, in the thymus toward an overall reduction of peripheral iNKT cells, unlike mainstream T cells. Furthermore, the effects of miR-155 over-expression on iNKT cell differentiation were cell autonomous. Finally, we identified Ets1 and ITK transcripts as relevant targets of miR-155 in iNKT cell differentiation. Altogether, these results demonstrate that a tight control of miR-155 expression is required for the development of iNKT cells. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1664-3224 |
Relation: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00140/full; https://doaj.org/toc/1664-3224 |
DOI: | 10.3389/fimmu.2015.00140 |
URL الوصول: | https://doaj.org/article/762c4ee46d5644ff865b6a14336f055a |
رقم الأكسشن: | edsdoj.762c4ee46d5644ff865b6a14336f055a |
قاعدة البيانات: | Directory of Open Access Journals |
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