دورية أكاديمية
Designing a Conserved Immunogenic Peptide Construct from the Nucleocapsid Protein of Puumala orthohantavirus
العنوان: | Designing a Conserved Immunogenic Peptide Construct from the Nucleocapsid Protein of Puumala orthohantavirus |
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المؤلفون: | Ayushi Sehgal, Diksha Sharma, Neha Kaushal, Yogita Gupta, Ekaterina Martynova, Emmanuel Kabwe, Sara Chandy, Albert Rizvanov, Svetlana Khaiboullina, Manoj Baranwal |
المصدر: | Viruses, Vol 16, Iss 7, p 1030 (2024) |
بيانات النشر: | MDPI AG, 2024. |
سنة النشر: | 2024 |
المجموعة: | LCC:Microbiology |
مصطلحات موضوعية: | orthohantavirus, hemorrhagic fever with renal syndrome (HFRS), Nucleocapsid protein, Multiepitope, HLA-peptide interactions, Microbiology, QR1-502 |
الوصف: | Puumala orthohantavirus (PUUV) is an emerging zoonotic virus endemic to Europe and Russia that causes nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome (HFRS). There are limited options for treatment and diagnosis of orthohantavirus infection, making the search for potential immunogenic candidates crucial. In the present work, various bioinformatics tools were employed to design conserved immunogenic peptides containing multiple epitopes of PUUV nucleocapsid protein. Eleven conserved peptides (90% conservancy) of the PUUV nucleocapsid protein were identified. Three conserved peptides containing multiple T and B cell epitopes were selected using a consensus epitope prediction algorithm. Molecular docking using the HPEP dock server demonstrated strong binding interactions between the epitopes and HLA molecules (ten alleles for each class I and II HLA). Moreover, an analysis of population coverage using the IEDB database revealed that the identified peptides have over 90% average population coverage across six continents. Molecular docking and simulation analysis reveal a stable interaction with peptide constructs of chosen immunogenic peptides and Toll-like receptor-4. These computational analyses demonstrate selected peptides’ immunogenic potential, which needs to be validated in different experimental systems. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1999-4915 |
Relation: | https://www.mdpi.com/1999-4915/16/7/1030; https://doaj.org/toc/1999-4915 |
DOI: | 10.3390/v16071030 |
URL الوصول: | https://doaj.org/article/7928dec4601546408a29f86d5fe9d799 |
رقم الأكسشن: | edsdoj.7928dec4601546408a29f86d5fe9d799 |
قاعدة البيانات: | Directory of Open Access Journals |
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