دورية أكاديمية
A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities
| العنوان: | A Novel Pectic Polysaccharide of Jujube Pomace: Structural Analysis and Intracellular Antioxidant Activities |
|---|---|
| المؤلفون: | Ximeng Lin, Keshan Liu, Sheng Yin, Yimin Qin, Peili Shen, Qiang Peng |
| المصدر: | Antioxidants, Vol 9, Iss 2, p 127 (2020) |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | polysaccharide, jujube pomace, structural analysis, antioxidant activity, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | After extraction from jujube pomace and purification by two columns (DEAE-Sepharose Fast Flow and Sepharcyl S-300), the structure of SAZMP4 was investigated by HPGPC, GC, FI-IR, GC-MS, NMR, SEM, and AFM. Analysis determined that SAZMP4 (Mw = 28.94 kDa) was a pectic polysaccharide mainly containing 1,4-linked GalA (93.48%) with side chains of 1,2,4-linked Rha and 1,3,5-linked Ara and terminals of 1-linked Rha and 1-linked Ara, which might be the homogalacturonan (HG) type with side chains of the RG-I type, corresponding to the results of NMR. In AFM and SEM images, self-assembly and aggregation of SAZMP4 were respectively observed indicating its structural features. The antioxidant activity of SAZMP4 against H2O2-induced oxidative stress in Caco-2 cells was determined by activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as malondialdehyde (MDA) and reactive oxygen species (ROS) levels, indicating SAZMP4 can be a natural antioxidant. Also, a better water retention capacity and thermal stability of SAZMP4 was observed based on DSC analysis, which could be applied in food industry as an additive. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2076-3921 |
| Relation: | https://www.mdpi.com/2076-3921/9/2/127; https://doaj.org/toc/2076-3921 |
| DOI: | 10.3390/antiox9020127 |
| URL الوصول: | https://doaj.org/article/dee7ce9381e449db88a644120c92b61d |
| رقم الأكسشن: | edsdoj.7ce9381e449db88a644120c92b61d |
| قاعدة البيانات: | Directory of Open Access Journals |
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