دورية أكاديمية
Immunogenic antitumor potential of Prakasine nanoparticles in zebrafish by gene expression stimulation
| العنوان: | Immunogenic antitumor potential of Prakasine nanoparticles in zebrafish by gene expression stimulation |
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| المؤلفون: | Prakash S. K. |
| المصدر: | Artificial Cells, Nanomedicine, and Biotechnology, Vol 51, Iss 1, Pp 41-56 (2023) |
| بيانات النشر: | Taylor & Francis Group, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Medical technology |
| مصطلحات موضوعية: | Nanoparticle, Prakasine nanoparticle, cytokine gene stimulation, cancer treatment, immunotherapy, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5 |
| الوصف: | AbstractIn this study, non-toxic mercury nanoparticle was synthesized as per “Prakash theory of metal drugs” and nanoparticle’s characters has been demonstrated by employing several nanotechnological tools including XPS, XRD, EDAX. The size of the Prakasine nanoparticles (PRK-NP) ranged from 90–100 nm, confirmed using TEM, SEM, DLS and along with zeta potential of −29.5 mV before storage and −8.5 mV after storage. The FTIR provided information regarding the nanoparticle capping and functional groups. The study was further elaborated for determining PRK-NPs toxicity, genotoxicity, in-vivo toxicity, immunological anti-tumour activity, immunogenicity potential, gene expression profiling and confirmed by MTT and apoptosis assays, cancer zebrafish model studies and WBC proliferation assay. PRK-NPs revealed no cytotoxicity where cell viability was observed 99% in L6 mouse fibroblasts and 99% in MCF-7 cell lines. Also, the cell viability was to be 89.47% at a very high concentration of 320 µg/ml in HEK 293 cells. The PRK-NPs significantly reduced the tumour in zebrafish at dose of 90 μg/g by up regulating IL-1α, IL-1β, IL-2-ITK, IL-6, IL-8, IL-12, TNF-α and IFN-γ, and down regulating IL-4, IL-5, IL-10 and TGF-β compared to untreated controls without any adverse effects and toxicity. Thus, the current study beholds anticipation PRK-NPs may play a vital role in therapeutic. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 21691401 2169-141X 2169-1401 |
| Relation: | https://doaj.org/toc/2169-1401; https://doaj.org/toc/2169-141X |
| DOI: | 10.1080/21691401.2023.2173217 |
| URL الوصول: | https://doaj.org/article/81882c7f2b9442b0b3825772f1f4b710 |
| رقم الأكسشن: | edsdoj.81882c7f2b9442b0b3825772f1f4b710 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 21691401 2169141X |
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| DOI: | 10.1080/21691401.2023.2173217 |