دورية أكاديمية
Design of artificial small regulatory trans-RNA for gene knockdown in Bacillus subtilis
| العنوان: | Design of artificial small regulatory trans-RNA for gene knockdown in Bacillus subtilis |
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| المؤلفون: | Guobin Yin, Anqi Peng, Luyao Zhang, Yang Wang, Guocheng Du, Jian Chen, Zhen Kang |
| المصدر: | Synthetic and Systems Biotechnology, Vol 8, Iss 1, Pp 61-68 (2023) |
| بيانات النشر: | KeAi Communications Co., Ltd., 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Biology (General) |
| مصطلحات موضوعية: | Gene knockdown, Posttranscriptional regulation, Small noncoding RNA, Synthetic biology, Cross-species, Bacillus subtilis, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5 |
| الوصف: | Bacillus subtilis as the Gram-positive model bacterium has been widely used in synthetic biology and biotechnology while the regulatory RNA tools for B. subtilis are still not fully explored. Here, a bottom-up approach is proposed for designing artificial trans-acting sRNAs. By engineering the intrinsic sRNA SR6, a minimized core scaffold structure consisting of an 8 bp stem, a 4 nt loop, and a 9 nt polyU tail was generated and proven to be sufficient for constructing sRNAs with strong repression activity (83%). Moreover, we demonstrate this artificial sRNA system functions well in an hfq-independent manner and also achieves strong repression efficiency in Escherichia coli (above 80%). A structure-based sRNA design principle was further developed for the automatic generation of custom sRNAs with this core scaffold but various sequences, which facilitates the manipulation and avoids structure disruption when fusing any base-pairing sequence. By applying these auto-designed sRNAs, we rapidly modified the cell morphology and biofilm formation, and regulated metabolic flux toward acetoin biosynthesis. This sRNA system with cross-species regulatory activities not only enriched the gene regulation toolkit in synthetic biology for B. subtilis and E. coli but also enhanced our understanding of trans-acting sRNAs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-805X |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405805X22001089; https://doaj.org/toc/2405-805X |
| DOI: | 10.1016/j.synbio.2022.11.003 |
| URL الوصول: | https://doaj.org/article/854596b518ca419bb0c494701ff66ff3 |
| رقم الأكسشن: | edsdoj.854596b518ca419bb0c494701ff66ff3 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2405805X |
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| DOI: | 10.1016/j.synbio.2022.11.003 |