دورية أكاديمية
Platelet-rich plasma promotes recruitment of macrophages in the process of tendon healing
| العنوان: | Platelet-rich plasma promotes recruitment of macrophages in the process of tendon healing |
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| المؤلفون: | Hirofumi Nishio, Yoshitomo Saita, Yohei Kobayashi, Tomoiku Takaku, Shin Fukusato, Sayuri Uchino, Takanori Wakayama, Hiroshi Ikeda, Kazuo Kaneko |
| المصدر: | Regenerative Therapy, Vol 14, Iss , Pp 262-270 (2020) |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine (General) LCC:Cytology |
| مصطلحات موضوعية: | Platelet-rich plasma, Macrophage, Immune response, Inflammatory cell balance, Tissue repair, Medicine (General), R5-920, Cytology, QH573-671 |
| الوصف: | Introduction: Researchers have investigated the use of platelet-rich plasma (PRP) therapy. However, the mechanisms through which PRP affects tissue repair remain unclear. We hypothesize that PRP promotes tissue repair through not only via direct manner on the local cells but also via indirect manner that encourage the recruitment of reparative cells such as macrophages (MPs), and it depends on the quality of PRP including the concentration of leukocytes. The aim of this study is to elucidate the actions of the MPs in the mechanisms of PRP on tissue repair processes. Methods: Leukocyte-rich (LR) PRP and leukocyte-poor (LP) PRP were prepared from 12-week-old C57BL6 mice. Full-thickness defects were created in central third of patellar tendons of 12-week-old C57BL/6 mice for histologic analysis (n = 36) and 12-week-old B6.129P-Cx3cr1tm1Litt/J mice for flow cytometry analysis (n = 108). B6.129P-Cx3cr1tm1Litt/J mouse is GFP-positive only in the MP-linage cells thus MPs recruited to the repair tissue can be distinguished whether it had originated from administrated PRP or recruited from host mouse. Mice were treated either with LR-PRP, LP-PRP, or without PRP (control group). Histological analyses were performed to evaluate the tendon healing using Bonar score as semi-quantitative histological scoring system. Flow cytometric analyses were performed to count the number of GFP-positive cells around repaired patellar tendon. In addition, the ratio of pro-inflammatory MPs (M1)/anti-inflammatory MPs (M2) were analyzed in those GFP-positive cells. The statistical analysis was performed using GraphPad Prism ver6. P values |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2352-3204 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2352320420300328; https://doaj.org/toc/2352-3204 |
| DOI: | 10.1016/j.reth.2020.03.009 |
| URL الوصول: | https://doaj.org/article/8b939abde52e402897560ea3e8570201 |
| رقم الأكسشن: | edsdoj.8b939abde52e402897560ea3e8570201 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23523204 |
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| DOI: | 10.1016/j.reth.2020.03.009 |