دورية أكاديمية
Cytomolecular Classification of Thyroid Nodules Using Fine-Needle Washes Aspiration Biopsies
| العنوان: | Cytomolecular Classification of Thyroid Nodules Using Fine-Needle Washes Aspiration Biopsies |
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| المؤلفون: | Giulia Capitoli, Isabella Piga, Vincenzo L’Imperio, Francesca Clerici, Davide Leni, Mattia Garancini, Gabriele Casati, Stefania Galimberti, Fulvio Magni, Fabio Pagni |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 8, p 4156 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | MALDI-MSI, thyroid carcinoma, proteomic analysis, diagnostic classification, fine-needle aspiration biopsies, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Fine-needle aspiration biopsies (FNA) represent the gold standard to exclude the malignant nature of thyroid nodules. After cytomorphology, 20–30% of cases are deemed “indeterminate for malignancy” and undergo surgery. However, after thyroidectomy, 70–80% of these nodules are benign. The identification of tools for improving FNA’s diagnostic performances is explored by matrix-assisted laser-desorption ionization mass spectrometry imaging (MALDI-MSI). A clinical study was conducted in order to build a classification model for the characterization of thyroid nodules on a large cohort of 240 samples, showing that MALDI-MSI can be effective in separating areas with benign/malignant cells. The model had optimal performances in the internal validation set (n = 70), with 100.0% (95% CI = 83.2–100.0%) sensitivity and 96.0% (95% CI = 86.3–99.5%) specificity. The external validation (n = 170) showed a specificity of 82.9% (95% CI = 74.3–89.5%) and a sensitivity of 43.1% (95% CI = 30.9–56.0%). The performance of the model was hampered in the presence of poor and/or noisy spectra. Consequently, restricting the evaluation to the subset of FNAs with adequate cellularity, sensitivity improved up to 76.5% (95% CI = 58.8–89.3). Results also suggest the putative role of MALDI-MSI in routine clinical triage, with a three levels diagnostic classification that accounts for an indeterminate gray zone of nodules requiring a strict follow-up. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/23/8/4156; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms23084156 |
| URL الوصول: | https://doaj.org/article/8cc6460e052740d3a412a56485512bd4 |
| رقم الأكسشن: | edsdoj.8cc6460e052740d3a412a56485512bd4 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms23084156 |