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Chlorpromazine protects against apoptosis induced by exogenous stimuli in the developing rat brain.

التفاصيل البيبلوغرافية
العنوان: Chlorpromazine protects against apoptosis induced by exogenous stimuli in the developing rat brain.
المؤلفون: Jing Wu, Rongrong Song, Wuqi Song, Yujun Li, Qingmeng Zhang, Yang Chen, Yingmei Fu, Wenjuan Fang, Jindong Wang, Zhaohua Zhong, Hong Ling, Liming Zhang, Fengmin Zhang
المصدر: PLoS ONE, Vol 6, Iss 7, p e21966 (2011)
بيانات النشر: Public Library of Science (PLoS), 2011.
سنة النشر: 2011
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: BACKGROUND: Chlorpromazine (CPZ), a commonly used antipsychotic drug, was found to play a neuroprotective role in various models of toxicity. However, whether CPZ has the potential to affect brain apoptosis in vivo is still unknown. The purpose of this study was to investigate the potential effect of CPZ on the apoptosis induced by exogenous stimuli. METHODOLOGY: The ethanol treated infant rat was utilized as a valid apoptotic model, which is commonly used and could trigger robust apoptosis in brain tissue. Prior to the induction of apoptosis by subcutaneous injection of ethanol, 7-day-old rats were treated with CPZ at several doses (5 mg/kg, 10 mg/kg and 20 mg/kg) by intraperitoneal injection. Apoptotic cells in the brain were measured using TUNEL analysis, and the levels of cleaved caspase-3, cytochrome c, the pro-apoptotic factor Bax and the anti-apoptotic factor Bcl-2 were assessed by immunostaining or western blot. FINDINGS: Compared to the group injected with ethanol only, the brains of the CPZ-pretreated rats had fewer apoptotic cells, lower expression of cleaved caspase-3, cytochrome c and Bax, and higher expression of Bcl-2. These results demonstrate that CPZ could prevent apoptosis in the brain by regulating the mitochondrial pathway. CONCLUSIONS: CPZ exerts an inhibitory effect on apoptosis induced by ethanol in the rat brain, intimating that it may offer a means of protecting nerve cells from apoptosis induced by exogenous stimuli.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3136481?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0021966
URL الوصول: https://doaj.org/article/91c66e35e996442fba2a50dc4b09dc3e
رقم الأكسشن: edsdoj.91c66e35e996442fba2a50dc4b09dc3e
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0021966