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Structural characterization of the extracellular polysaccharide from Vibrio cholerae O1 El-Tor.

التفاصيل البيبلوغرافية
العنوان: Structural characterization of the extracellular polysaccharide from Vibrio cholerae O1 El-Tor.
المؤلفون: Fitnat Yildiz, Jiunn Fong, Irina Sadovskaya, Thierry Grard, Evgeny Vinogradov
المصدر: PLoS ONE, Vol 9, Iss 1, p e86751 (2014)
بيانات النشر: Public Library of Science (PLoS), 2014.
سنة النشر: 2014
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: The ability to form biofilms is important for environmental survival, transmission, and infectivity of Vibrio cholerae, the causative agent of cholera in humans. To form biofilms, V. cholerae produces an extracellular matrix composed of proteins, nucleic acids and a glycoconjugate, termed Vibrio exopolysaccharide (VPS). Here, we present the data on isolation and characterization of the polysaccharide part of the VPS (VPS-PS), which has the following structure: -4)-α-GulpNAcAGly3OAc-(1-4)-β-D-Glcp-(1-4)-α-Glcp-(1-4)-α-D-Galp-(1- where α-D-Glc is partially (∼20%) replaced with α-D-GlcNAc. α-GulNAcAGly is an amide between 2-acetamido-2-deoxy-α-guluronic acid and glycine. Apparently, the polysaccharide is bound to a yet unidentified component, which gives it high viscosity and completely suppresses any NMR signals belonging to the sugar chains of the VPS. The only reliable method to remove this component at present is a treatment of the whole glycoconjugate with concentrated hydrochloric acid.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3901696?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0086751
URL الوصول: https://doaj.org/article/936ff321bdbf4fd48ef7421e760cc942
رقم الأكسشن: edsdoj.936ff321bdbf4fd48ef7421e760cc942
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0086751