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Establishment and application of a quadruple real-time RT-PCR for detecting avian metapneumovirus.

التفاصيل البيبلوغرافية
العنوان: Establishment and application of a quadruple real-time RT-PCR for detecting avian metapneumovirus.
المؤلفون: Suchun Wang, Nan Jiang, Lijian Jiang, Qingye Zhuang, Qiong Chen, Guangyu Hou, Zhiyu Xiao, Ran Zhao, Yang Li, Chenglong Zhao, Fuyou Zhang, Jianmin Yu, Jinping Li, Hualei Liu, Fuliang Sun, Kaicheng Wang
المصدر: PLoS ONE, Vol 17, Iss 6, p e0270708 (2022)
بيانات النشر: Public Library of Science (PLoS), 2022.
سنة النشر: 2022
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: In order to develop an appropriate method for high-throughput detection of avian metapneumovirus, a quadruple real-time reverse-transcription polymerase chain reaction assay was established with four pairs of specific primers and four specific probes based on the G or M gene of aMPV-A, aMPV-B, aMPV-C and aMPV-D. Its specificity and sensitivity were evaluated, and clinical samples were tested by the method. The results showed that all the four subgroups of avian metapneumovirus can be detected in the quadruple real-time RT-PCR assay simultaneously, with a detection limit of 100-1000 cRNA copies/reaction. The other common poultry viruses were negative. In the avian clinical sample detection, 39 out of 1920 clinical samples collected from 8 provinces were positive. Compared with published RT-PCR assays, the κ value of the quadruple real-time RT-PCR assay in 1920 avian clinical samples was 1.000 (P < 0.001). The established method could be used for the rapid detection of the four subgroups of avian metapneumovirus with high specificity and high sensitivity.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0270708
URL الوصول: https://doaj.org/article/99c122ee1d024b25a7b2a543e6c6a8f7
رقم الأكسشن: edsdoj.99c122ee1d024b25a7b2a543e6c6a8f7
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0270708