دورية أكاديمية
Overexpression of Wnt7a enhances radiosensitivity of non-small-cell lung cancer via the Wnt/JNK pathway
| العنوان: | Overexpression of Wnt7a enhances radiosensitivity of non-small-cell lung cancer via the Wnt/JNK pathway |
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| المؤلفون: | Pingping Ai, Xianhua Xu, Shijie Xu, Zhixia Wei, Shun Tan, Junzhe Li |
| المصدر: | Biology Open, Vol 9, Iss 6 (2020) |
| بيانات النشر: | The Company of Biologists, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | non-small-cell lung cancer (nsclc), wnt7a, radiosensitivity, wnt/jnk pathway, Science, Biology (General), QH301-705.5 |
| الوصف: | The Wingless-type protein 7a (Wnt7a) plays an antiproliferative role in non-small-cell lung cancer (NSCLC). Previous studies have indicated that Wnt7a expression was downregulated in radiation-resistant NSCLC cells. However, little is known about its biological functions and molecular mechanisms in radiosensitivity of NSCLC. Thus, NSCLC cell proliferation and apoptosis in response to Wnt7a overexpression and/or radiation were determined by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl-tertazolium bromide (MTT) assay and flow cytometry, respectively. The activation of the Wnt/cJun N-terminal kinase (JNK) and Wnt/β-catenin signaling pathways were further examined by western blot in NSCLC cell lines H1650 and A549. Wnt7a overexpression combined with radiation-inhibited cell proliferation and induced apoptosis in NSCLC cell lines compared to Wnt7a overexpression or radiotherapy alone. In addition, the phosphorylation of JNK, but not β-catenin, was congruent with the changes in Wnt7a overexpression and/or radiation. Moreover, the Wnt/JNK pathway could induce the apoptosis of NSCLC cells through the mitochondrial pathway. Inhibition of the Wnt/JNK signaling pathway by SP600125, a JNK inhibitor, contributed to proliferation induction in NSCLC cells. Taken together, these results showed that Wnt7a overexpression sensitized NSCLC cell lines to radiotherapy through the Wnt/JNK signaling pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2046-6390 |
| Relation: | http://bio.biologists.org/content/9/6/bio050575; https://doaj.org/toc/2046-6390 |
| DOI: | 10.1242/bio.050575 |
| URL الوصول: | https://doaj.org/article/db0512c30afd47d4861c61f767b0cbea |
| رقم الأكسشن: | edsdoj.b0512c30afd47d4861c61f767b0cbea |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20466390 |
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| DOI: | 10.1242/bio.050575 |