دورية أكاديمية
Expression and Localization Profiles of Tight Junction Proteins in Immune Cells Depend on Their Activation Status
| العنوان: | Expression and Localization Profiles of Tight Junction Proteins in Immune Cells Depend on Their Activation Status |
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| المؤلفون: | Lena Voges, Franziska Weiß, Ana-Teresa Branco, Michael Fromm, Susanne M. Krug |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 9, p 4861 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | immune cells, tight junction proteins, claudins, TAMP, angulin, LSR, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | The ability of the immune system to combat pathogens relies on processes like antigen sampling by dendritic cells and macrophages migrating through endo- and epithelia or penetrating them with their dendrites. In addition, other immune cell subtypes also migrate through the epithelium after activation. For paracellular migration, interactions with tight junctions (TJs) are necessary, and previous studies reported TJ protein expression in several immune cells. Our investigation aimed to characterize, in more detail, the expression profiles of TJ proteins in different immune cells in both naïve and activated states. The mRNA expression analysis revealed distinct expression patterns for TJ proteins, with notable changes, mainly increases, upon activation. At the protein level, LSR appeared predominant, being constitutively present in naïve cell membranes, suggesting roles as a crucial interaction partner. Binding experiments suggested the presence of claudins in the membrane only after stimulation, and claudin-8 translocation to the membrane occurred after stimulation. Our findings suggest a dynamic TJ protein expression in immune cells, implicating diverse functions in response to stimulation, like interaction with TJ proteins or regulatory roles. While further analysis is needed to elucidate the precise roles of TJ proteins, our findings indicate important non-canonical functions of TJ proteins in immune response. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/25/9/4861; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms25094861 |
| URL الوصول: | https://doaj.org/article/b0d1b7dfbe294f6d993c76d2eed0fd2b |
| رقم الأكسشن: | edsdoj.b0d1b7dfbe294f6d993c76d2eed0fd2b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms25094861 |