دورية أكاديمية
The Catalytic Activity of Human REV1 on Undamaged and Damaged DNA
| العنوان: | The Catalytic Activity of Human REV1 on Undamaged and Damaged DNA |
|---|---|
| المؤلفون: | Anastasia D. Stolyarenko, Anna A. Novikova, Evgeniy S. Shilkin, Valentin A. Poltorachenko, Alena V. Makarova |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 7, p 4107 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | REV1, DNA polymerase, translesion DNA synthesis, AP site, cisplatin intrastrand crosslink, Hoogsteen interactions, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Eukaryotic REV1 serves as a scaffold protein for the coordination of DNA polymerases during DNA translesion synthesis. Besides this structural role, REV1 is a Y-family DNA polymerase with its own distributive deoxycytidyl transferase activity. However, data about the accuracy and efficiency of DNA synthesis by REV1 in the literature are contrasting. Here, we expressed and purified the full-length human REV1 from Saccharomyces cerevisiae and characterized its activity on undamaged DNA and a wide range of damaged DNA templates. We demonstrated that REV1 carried out accurate synthesis opposite 8-oxoG and O6-meG with moderate efficiency. It also replicated thymine glycol surprisingly well in an error-prone manner, but was blocked by the intrastrand 1,2-GG cisplatin crosslink. By using the 1,N6-ethenoadenine and 7-deaza-adenine lesions, we have provided biochemical evidence of the importance for REV1 functioning of the Hoogsteen face of template A, the second preferable template after G. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/25/7/4107; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms25074107 |
| URL الوصول: | https://doaj.org/article/db2c3bfc387a489d884330840bce02cc |
| رقم الأكسشن: | edsdoj.b2c3bfc387a489d884330840bce02cc |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
|---|---|
| DOI: | 10.3390/ijms25074107 |