دورية أكاديمية
Environmental DNA dynamics of three species of unionid freshwater mussels
| العنوان: | Environmental DNA dynamics of three species of unionid freshwater mussels |
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| المؤلفون: | Dannise V. Ruiz‐Ramos, Nathan L. Thompson, Catherine A. Richter, Megan C. Voshage, Theresa M. Schreier, Christopher M. Merkes, Katy E. Klymus |
| المصدر: | Environmental DNA, Vol 6, Iss 2, Pp n/a-n/a (2024) |
| بيانات النشر: | Wiley, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Environmental sciences LCC:Microbial ecology |
| مصطلحات موضوعية: | degradation rates, environmental DNA, freshwater mussels, shedding rates, Unionidae, Environmental sciences, GE1-350, Microbial ecology, QR100-130 |
| الوصف: | Abstract North American freshwater mussels are of special conservation concern due to their high endemism and the multiple anthropogenic stressors affecting them. Of the over 300 species in North America, nearly one third of these species are federally listed as threatened or endangered. Environmental DNA (eDNA) analysis has been successful in detecting freshwater mussels and could aid in monitoring their populations. Production and degradation rates of eDNA for the species of interest are needed to inform interpretation of eDNA detections, allow possible modeling of relative abundance and population location, and aid in mussel conservation through population identification. Here, we designed and tested qPCR assays for three freshwater mussel species, mucket (Ortmanniana ligamentina), fatmucket (Lampsilis siliquoidea), and the federally endangered spectaclecase (Cumberlandia monodonta). We performed laboratory experiments under controlled conditions to measure eDNA shedding and degradation rates for each species. Different biomasses, temperatures, and food regimens were tested independently to determine if these factors influence the amount of DNA produced by the mussels. Degradation rates of eDNA were measured from experimental tank water after mussels were removed. Overall, we observed low eDNA shedding rates for freshwater mussels compared to previous studies of fish eDNA shedding rates. Furthermore, temperature and feeding showed limited or no significant effects in the species studied. Environmental DNA degradation rates were consistent with those reported in the literature for other taxa. Collectively, our results will be useful for designing eDNA monitoring studies, modeling eDNA dispersal, and interpreting eDNA results to help inform freshwater mussel conservation efforts. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2637-4943 |
| Relation: | https://doaj.org/toc/2637-4943 |
| DOI: | 10.1002/edn3.543 |
| URL الوصول: | https://doaj.org/article/b58c7ea18c934bf0955c09a5931b769c |
| رقم الأكسشن: | edsdoj.b58c7ea18c934bf0955c09a5931b769c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26374943 |
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| DOI: | 10.1002/edn3.543 |