دورية أكاديمية
Enhanced Expression of Anti-CD19 Chimeric Antigen Receptor in piggyBac Transposon-Engineered T Cells
| العنوان: | Enhanced Expression of Anti-CD19 Chimeric Antigen Receptor in piggyBac Transposon-Engineered T Cells |
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| المؤلفون: | Daisuke Morita, Nobuhiro Nishio, Shoji Saito, Miyuki Tanaka, Nozomu Kawashima, Yusuke Okuno, Satoshi Suzuki, Kazuyuki Matsuda, Yasuhiro Maeda, Matthew H. Wilson, Gianpietro Dotti, Cliona M. Rooney, Yoshiyuki Takahashi, Yozo Nakazawa |
| المصدر: | Molecular Therapy: Methods & Clinical Development, Vol 8, Iss C, Pp 131-140 (2018) |
| بيانات النشر: | Elsevier, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Genetics LCC:Cytology |
| مصطلحات موضوعية: | chimeric antigen receptor, non-viral vector, piggyBac, transposon, virus-specific antigens, acute lymphoblastic leukemia, refractory, Genetics, QH426-470, Cytology, QH573-671 |
| الوصف: | Adoptive T cell therapy using chimeric antigen receptor (CAR)-modified T cells is a promising cancer immunotherapy. We previously developed a non-viral method of gene transfer into T cells using a piggyBac transposon system to improve the cost-effectiveness of CAR-T cell therapy. Here, we have further improved our technology by a novel culture strategy to increase the transfection efficiency and to reduce the time of T cell manufacturing. Using a CH2CH3-free CD19-specific CAR transposon vector and combining irradiated activated T cells (ATCs) as feeder cells and virus-specific T cell receptor (TCR) stimulation, we achieved 51.4% ± 14% CAR+ T cells and 2.8-fold expansion after 14 culture days. Expanded CD19.CAR-T cells maintained a significant fraction of CD45RA+CCR7+ T cells and demonstrated potent antitumor activity against CD19+ leukemic cells both in vitro and in vivo. Therefore, piggyBac-based gene transfer may provide an alternative to viral gene transfer for CAR-T cell therapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2329-0501 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2329050117301286; https://doaj.org/toc/2329-0501 |
| DOI: | 10.1016/j.omtm.2017.12.003 |
| URL الوصول: | https://doaj.org/article/b87077edcc27440da345a159cfeca963 |
| رقم الأكسشن: | edsdoj.b87077edcc27440da345a159cfeca963 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23290501 |
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| DOI: | 10.1016/j.omtm.2017.12.003 |