دورية أكاديمية
Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina
العنوان: | Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina |
---|---|
المؤلفون: | Diana Pauly, Divyansh Agarwal, Nicholas Dana, Nicole Schäfer, Josef Biber, Kirsten A. Wunderlich, Yassin Jabri, Tobias Straub, Nancy R. Zhang, Avneesh K. Gautam, Bernhard H.F. Weber, Stefanie M. Hauck, Mijin Kim, Christine A. Curcio, Dwight Stambolian, Mingyao Li, Antje Grosche |
المصدر: | Cell Reports, Vol 29, Iss 9, Pp 2835-2848.e4 (2019) |
بيانات النشر: | Elsevier, 2019. |
سنة النشر: | 2019 |
المجموعة: | LCC:Biology (General) |
مصطلحات موضوعية: | Biology (General), QH301-705.5 |
الوصف: | Summary: Complement dysregulation is a feature of many retinal diseases, yet mechanistic understanding at the cellular level is limited. Given this knowledge gap about which retinal cells express complement, we performed single-cell RNA sequencing on ∼92,000 mouse retinal cells and validated our results in five major purified retinal cell types. We found evidence for a distributed cell-type-specific complement expression across 11 cell types. Notably, Müller cells are the major contributor of complement activators c1s, c3, c4, and cfb. Retinal pigment epithelium (RPE) mainly expresses cfh and the terminal complement components, whereas cfi and cfp transcripts are most abundant in neurons. Aging enhances c1s, cfb, cfp, and cfi expression, while cfh expression decreases. Transient retinal ischemia increases complement expression in microglia, Müller cells, and RPE. In summary, we report a unique complement expression signature for murine retinal cell types suggesting a well-orchestrated regulation of local complement expression in the retinal microenvironment. : Overshooting complement activity contributes to retinal degeneration. Pauly et al. demonstrate a distinct complement expression profile of retinal cell types that changes with aging and during retinal degeneration. This prompts the intriguing concept of a local retinal complement activation possibly independent of the systemic components typically produced by the liver. keywords: complement expression, retina, eye, classical, alternative, lectin, Müller cell, neuron, ischemia, age-dependent |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2211-1247 |
Relation: | http://www.sciencedirect.com/science/article/pii/S2211124719314068; https://doaj.org/toc/2211-1247 |
DOI: | 10.1016/j.celrep.2019.10.084 |
URL الوصول: | https://doaj.org/article/bac62f836d3541a2a490fbcf404c3aeb |
رقم الأكسشن: | edsdoj.bac62f836d3541a2a490fbcf404c3aeb |
قاعدة البيانات: | Directory of Open Access Journals |
كن أول من يترك تعليقا!