دورية أكاديمية
Validation of internal reference genes for relative quantitation studies of gene expression in human laryngeal cancer
| العنوان: | Validation of internal reference genes for relative quantitation studies of gene expression in human laryngeal cancer |
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| المؤلفون: | Xiaofeng Wang, Jinting He, Wei Wang, Ming Ren, Sujie Gao, Guanjie Zhao, Jincheng Wang, Qiwei Yang |
| المصدر: | PeerJ, Vol 4, p e2763 (2016) |
| بيانات النشر: | PeerJ Inc., 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Medicine LCC:Biology (General) |
| مصطلحات موضوعية: | Housekeeping gene, Reverse transcription quantitative polymerase chain reaction, Human laryngeal cancer, Normalization, Expression stability, Medicine, Biology (General), QH301-705.5 |
| الوصف: | Background The aim of this study was to determine the expression stabilities of 12 common internal reference genes for the relative quantitation analysis of target gene expression performed by reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) in human laryngeal cancer. Methods Hep-2 cells and 14 laryngeal cancer tissue samples were investigated. The expression characteristics of 12 internal reference gene candidates (18S rRNA, GAPDH, ACTB, HPRT1, RPL29, HMBS, PPIA, ALAS1, TBP, PUM1, GUSB, and B2M) were assessed by RT-qPCR. The data were analyzed by three commonly used software programs: geNorm, NormFinder, and BestKeeper. Results The use of the combination of four internal reference genes was more appropriate than the use of a single internal reference gene. The optimal combination was PPIA + GUSB + RPL29 + HPRT1 for both the cell line and tissues; while the most appropriate combination was GUSB + RPL29 + HPRT1 + HMBS for the tissues. Conclusions Our recommended internal reference genes may improve the accuracy of relative quantitation analysis of target gene expression performed by the RT-qPCR method in further gene expression research on laryngeal tumors. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2167-8359 |
| Relation: | https://peerj.com/articles/2763.pdf; https://peerj.com/articles/2763/; https://doaj.org/toc/2167-8359 |
| DOI: | 10.7717/peerj.2763 |
| URL الوصول: | https://doaj.org/article/f5a057493b7d4a8487e1f6a6e80add02 |
| رقم الأكسشن: | edsdoj.f5a057493b7d4a8487e1f6a6e80add02 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21678359 |
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| DOI: | 10.7717/peerj.2763 |