دورية أكاديمية
Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo
| العنوان: | Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo |
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| المؤلفون: | Ziwei Lin, Tiantian Gan, Yanzhen Huang, Lijun Bao, Shuang Liu, Xiaopeng Cui, Hexin Wang, Feng Jiao, Minjuan Zhang, Chao Su, Yonghua Qian |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 14, p 7694 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | mulberry leaf, flavonoids, anti-inflammatory, LC-MS, ulcerative colitis, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Mulberry (Morus alba L.) is a flowering tree traditionally used in Chinese herbal medicine. Mulberry leaf flavonoids (MLFs) have been reported to exert important anti-inflammatory and antioxidant properties. The purpose of this study was to select the MLF with the best anti-inflammatory and antioxidative activities from MLFs eluted by different ethanol concentrations (30%, 50%, and 75%) and explore its pharmacological properties. Three types of MLFs inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and inflammatory cytokines in lipopolysaccharide (LPS)-induced RAW 264.7 cells. All MLFs boosted the antioxidative capacity by decreasing the reactive oxygen species (ROS) production and the scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and improving the metal ion chelating activity and reducing power. The results revealed that the MLFs eluted by 30% ethanol exhibited the best anti-inflammatory and antioxidative activities. A nontargeted metabolomic analysis was used to analyze 24 types of differential flavonoids between the MLFs. Quercetin, kaempferol, and their derivatives in 30%MLF were more abundant than the other two MLFs. Furthermore, we evaluated the pharmacological activities of 30%MLF in dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) mice. The 30%MLF could alleviate the clinical symptoms, reduce the secretion of inflammatory cytokines, and inhibit the activation of the inflammatory pathway in DSS-induced colitis mice. This study will provide valuable information for the development of MLFs eluted by 30% ethanol as a functional food. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/23/14/7694; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms23147694 |
| URL الوصول: | https://doaj.org/article/f6bc6e4efd7f490d9654184b33cda7c7 |
| رقم الأكسشن: | edsdoj.f6bc6e4efd7f490d9654184b33cda7c7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms23147694 |