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Identification of CD38, CD97, and CD278 on the HIV surface using a novel flow virometry screening assay

التفاصيل البيبلوغرافية
العنوان: Identification of CD38, CD97, and CD278 on the HIV surface using a novel flow virometry screening assay
المؤلفون: Jonathan Burnie, Claire Fernandes, Deepa Chaphekar, Danlan Wei, Shubeen Ahmed, Arvin Tejnarine Persaud, Nawrah Khader, Claudia Cicala, James Arthos, Vera A. Tang, Christina Guzzo
المصدر: Scientific Reports, Vol 13, Iss 1, Pp 1-16 (2023)
بيانات النشر: Nature Portfolio, 2023.
سنة النشر: 2023
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Abstract While numerous cellular proteins in the HIV envelope are known to alter virus infection, methodology to rapidly phenotype the virion surface in a high throughput, single virion manner is lacking. Thus, many human proteins may exist on the virion surface that remain undescribed. Herein, we developed a novel flow virometry screening assay to discover new proteins on the surface of HIV particles. By screening a CD4+ T cell line and its progeny virions, along with four HIV isolates produced in primary cells, we discovered 59 new candidate proteins in the HIV envelope that were consistently detected across diverse HIV isolates. Among these discoveries, CD38, CD97, and CD278 were consistently present at high levels on virions when using orthogonal techniques to corroborate flow virometry results. This study yields new discoveries about virus biology and demonstrates the utility and feasibility of a novel flow virometry assay to phenotype individual virions.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
Relation: https://doaj.org/toc/2045-2322
DOI: 10.1038/s41598-023-50365-0
URL الوصول: https://doaj.org/article/f9c07fc34cf74f4db67a89f694372680
رقم الأكسشن: edsdoj.f9c07fc34cf74f4db67a89f694372680
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-023-50365-0