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Expanding the Use of a Fluorogenic Method to Determine Activity and Mode of Action of Bacillus thuringiensis Bacteriocins Against Gram-Positive and Gram-Negative Bacteria

التفاصيل البيبلوغرافية
العنوان: Expanding the Use of a Fluorogenic Method to Determine Activity and Mode of Action of Bacillus thuringiensis Bacteriocins Against Gram-Positive and Gram-Negative Bacteria
المؤلفون: Norma M. de la Fuente-Salcido, J. Eleazar Barboza-Corona, A. N. Espino Monzón, R. D. Pacheco Cano, N. Balagurusamy, Dennis K. Bideshi, Rubén Salcedo-Hernández
المصدر: The Scientific World Journal, Vol 2012 (2012)
بيانات النشر: Wiley, 2012.
سنة النشر: 2012
المجموعة: LCC:Technology
LCC:Medicine
LCC:Science
مصطلحات موضوعية: Technology, Medicine, Science
الوصف: Previously we described a rapid fluorogenic method to measure the activity of five bacteriocins produced by Mexican strains of Bacillus thuringiensis against B. cereus 183. Here we standardize this method to efficiently determine the activity of bacteriocins against both Gram-positive and Gram-negative bacteria. It was determined that the crucial parameter required to obtain reproducible results was the number of cells used in the assay, that is, ~4 × 108 cell/mL and ~7 × 108 cell/mL, respectively, for target Gram-positive and Gram-negative bacteria. Comparative analyses of the fluorogenic and traditional well-diffusion assays showed correlation coefficients of 0.88 to 0.99 and 0.83 to 0.99, respectively, for Gram-positive and Gram-negative bacteria. The fluorogenic method demonstrated that the five bacteriocins of B. thuringiensis have bacteriolytic and bacteriostatic activities against all microorganisms tested, including clinically significant bacteria such as Listeria monocytogenes, Proteus vulgaris, and Shigella flexneri reported previously to be resistant to the antimicrobials as determined using the well-diffusion protocol. These results demonstrate that the fluorogenic assay is a more sensitive, reliable, and rapid method when compared with the well-diffusion method and can easily be adapted in screening protocols for bacteriocin production by other microorganisms.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1537-744X
Relation: https://doaj.org/toc/1537-744X
DOI: 10.1100/2012/503269
URL الوصول: https://doaj.org/article/fc391bba6ffd42a6aad4233afde47973
رقم الأكسشن: edsdoj.fc391bba6ffd42a6aad4233afde47973
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:1537744X
DOI:10.1100/2012/503269