دورية أكاديمية
New MIP-based Electrochemical Device for On-site Detection of Glucose Oxidase
| العنوان: | New MIP-based Electrochemical Device for On-site Detection of Glucose Oxidase |
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| المؤلفون: | Tânia S. C. R. REBELO, Inês M. MIRANDA, Laura SOUSA, José A. RIBEIRO |
| المصدر: | Sensors & Transducers, Vol 246, Iss 7, Pp 16-22 (2020) |
| بيانات النشر: | IFSA Publishing, S.L., 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Technology (General) |
| مصطلحات موضوعية: | glucose oxidase (god), caffeic acid (caf), electrochemical biosensor, molecularly imprinted polymer (mip), carbon, screen-printed electrode (spe), Technology (General), T1-995 |
| الوصف: | The measurement of protein concentration is a fundamental assay in diverse areas, such as medicine, environmental control and also in food industry, for monitoring enzyme content in consumer goods. In this work, the design and synthesis of artificial receptors, based on molecular imprinting (MI) technology, is described for the development of a simple and efficient electrochemical MIP-based biosensor for detection of glucose oxidase (GOD). Disposable and cost-effective chips, consisting of carbon screen-printed electrodes (C-SPEs), were used to develop the portable electrochemical devices for on-site monitoring of GOD in industrial context. MIP receptor films were created by electropolymerization of caffeic acid (CAF), in the presence of GOD as template biomolecule, at the carbon electrode surface, followed by enzyme wash-out from the polymeric matrix in order to create the specific binding sites. After that, specific binding properties between the MIP film surface and the template protein were observed. The developed MIP biosensor exhibited a very wide working concentration range, between 10-9 to 10-3 g L- 1, with a limit of detection (LOD) of 1.0´10-10 g L-1. Moreover, selectivity studies were performed against other proteins (amylase and myoglobin) revealing that the MIP surface has high specificity for GOD. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2306-8515 1726-5479 |
| Relation: | https://sensorsportal.com/HTML/DIGEST/november_2020/Vol_246/P_3182.pdf; https://doaj.org/toc/2306-8515; https://doaj.org/toc/1726-5479 |
| URL الوصول: | https://doaj.org/article/dfc43846fcdf4357b1eb86b7af42adf8 |
| رقم الأكسشن: | edsdoj.fc43846fcdf4357b1eb86b7af42adf8 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23068515 17265479 |
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