تقرير
Peptide conjugates with polyaromatic hydrocarbons can benefit the activity of catalytic RNAs
| العنوان: | Peptide conjugates with polyaromatic hydrocarbons can benefit the activity of catalytic RNAs |
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| المؤلفون: | Kevin J. Sweeney, Tommy Le, Micaella Z. Jorge, Joan G. Schellinger, Luke J. Leman, Ulrich F. Müller |
| المصدر: | Chemical Science. 37 |
| بيانات النشر: | United States: NASA Center for Aerospace Information (CASI), 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Chemistry and Materials (General) |
| الوصف: | Early stages of life likely employed catalytic RNAs (ribozymes) in many functions that are today filled by proteins. However, the earliest life forms must have emerged from heterogenous chemical mixtures, which included amino acids, short peptides, and many other compounds. Here we explored whether the presence of short peptides can help the emergence of catalytic RNAs. To do this, we conducted an in vitro selection for catalytic RNAs from randomized sequence in the presence of ten different peptides with a prebiotically plausible length of eight amino acids. This in vitro selection generated dozens of ribozymes, one of them with ∼900-fold higher activity in the presence of one specific peptide. Unexpectedly, the beneficial peptide had retained its N-terminal Fmoc protection group, and this group was required to benefit ribozyme activity. The same, or higher benefit resulted from peptide conjugates with prebiotically plausible polyaromatic hydrocarbons (PAHs) such as fluorene and naphthalene. This shows that PAH-peptide conjugates can act as potent cofactors to enhance ribozyme activity. The results are discussed in the context of the origin of life. |
| نوع الوثيقة: | Report |
| اللغة: | English |
| تدمد: | 2041-6539 2041-6520 |
| DOI: | 10.1039/d3sc03540a |
| URL الوصول: | https://ntrs.nasa.gov/citations/20240000671 |
| ملاحظات: | NNX16AJ27G |
| رقم الأكسشن: | edsnas.20240000671 |
| قاعدة البيانات: | NASA Technical Reports |
Full Text Finder | تدمد: | 20416539 20416520 |
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| DOI: | 10.1039/d3sc03540a |