مورد إلكتروني
Crystal structures of Trypanosoma brucei sterol 14alpha-demethylase and implications for selective treatment of human infections.
| العنوان: | Crystal structures of Trypanosoma brucei sterol 14alpha-demethylase and implications for selective treatment of human infections. |
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| المؤلفون: | Lepesheva, Galina I, Park, Hee-Won, Hargrove, Tatiana Y, Vanhollebeke, Benoît, Wawrzak, Zdzislaw, Harp, Joel M, Sundaramoorthy, Munirathinam, Nes, W David, Pays, Etienne, Chaudhuri, Minu, Villalta, Fernando, Waterman, Michael R |
| المصدر: | The Journal of biological chemistry, 285 (3 |
| بيانات النشر: | 2010-01 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Sterol 14alpha-demethylase (14DM, the CYP51 family of cytochrome P450) is an essential enzyme in sterol biosynthesis in eukaryotes. It serves as a major drug target for fungal diseases and can potentially become a target for treatment of human infections with protozoa. Here we present 1.9 A resolution crystal structures of 14DM from the protozoan pathogen Trypanosoma brucei, ligand-free and complexed with a strong chemically selected inhibitor N-1-(2,4-dichlorophenyl)-2-(1H-imidazol-1-yl)ethyl)-4-(5-phenyl-1,3,4-oxadi-azol-2-yl)benzamide that we previously found to produce potent antiparasitic effects in Trypanosomatidae. This is the first structure of a eukaryotic microsomal 14DM that acts on sterol biosynthesis, and it differs profoundly from that of the water-soluble CYP51 family member from Mycobacterium tuberculosis, both in organization of the active site cavity and in the substrate access channel location. Inhibitor binding does not cause large scale conformational rearrangements, yet induces unanticipated local alterations in the active site, including formation of a hydrogen bond network that connects, via the inhibitor amide group fragment, two remote functionally essential protein segments and alters the heme environment. The inhibitor binding mode provides a possible explanation for both its functionally irreversible effect on the enzyme activity and its selectivity toward the 14DM from human pathogens versus the human 14DM ortholog. The structures shed new light on 14DM functional conservation and open an excellent opportunity for directed design of novel antiparasitic drugs. Journal Article Research Support, N.I.H. Extramural Research Support, Non-U.S. Gov't SCOPUS: ar.j info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Amino Acid Sequence, Benzamides -- chemistry, Benzamides -- metabolism, Benzamides -- pharmacology, Benzamides -- therapeutic use, Biocatalysis, Crystallography, X-Ray, Cytochrome P-450 Enzyme System -- antagonists & inhibitors, Cytochrome P-450 Enzyme System -- chemistry, Cytochrome P-450 Enzyme System -- metabolism, Drug Design, Enzyme Inhibitors -- chemistry, Enzyme Inhibitors -- metabolism, Enzyme Inhibitors -- pharmacology, Enzyme Inhibitors -- therapeutic use, Humans, Ligands, Microsomes -- enzymology, Models, Molecular, Protein Conformation, Sterols -- biosynthesis, Substrate Specificity, Trypanocidal Agents -- chemistry, Trypanocidal Agents -- metabolism, Trypanocidal Agents -- pharmacology, Trypanocidal Agents -- therapeutic use, Trypanosoma brucei brucei -- drug effects, Trypanosoma brucei brucei -- enzymology, Trypanosomiasis, African -- drug therapy, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content 2 full-text file(s): info:eu-repo/semantics/openAccess | info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | 2 full-text file(s): application/pdf | application/pdf English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/51605 uri/info:doi/10.1074/jbc.M109.067470 uri/info:pii/M109.067470 uri/info:pmid/19923211 uri/info:scp/76249100348 764594131 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.ocn764594131 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |