مورد إلكتروني
Methotrexate inhibits LPS-induced tumor necrosis factor production in vivo.
| العنوان: | Methotrexate inhibits LPS-induced tumor necrosis factor production in vivo. |
|---|---|
| المؤلفون: | Durez, Patrick, Appelboom, Thierry, Vray, B, Pira, C, Goldman, Michel |
| المصدر: | European cytokine network, 9 (4 |
| بيانات النشر: | 1998-12 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | We investigated the effects of methotrexate (MTX) on the production of tumor necrosis factor (TNF), nitric oxide (NO) and interleukin-10 (IL-10) in vivo. Administration of low doses of MTX (450 mug/kg once a week for 4 weeks) in Balb/c mice resulted in a significant inhibition of the systemic release of TNF and NO upon LPS challenge, whereas the production of IL-10 remained unchanged. These anti-inflammatory effects of MTX were exerted at the macrophage level since peritoneal macrophages from mice, injected for 4 weeks with MTX produced lower levels of TNF than cells from control mice upon LPS stimulation ex vivo. In parallel, we found that MTX-treated mice were less susceptible to a lethal challenge with LPS and D-galactosamine. MTX did not inhibit TNF release after injection of Concanavalin A (ConA) or anti-CD3 monoclonal antibody (mAb), indicating that MTX does not inhibit TNF production at the T cell level. We conclude that repeated administration of a low dose of MTX in mice is associated with a decreased production of TNF by macrophages upon LPS activation. In Vitro Journal Article Research Support, Non-U.S. Gov't info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Animals, Antibodies, Monoclonal -- pharmacology, Antigens, CD3 -- immunology, Antirheumatic Agents -- pharmacology, Concanavalin A -- pharmacology, Galactosamine -- pharmacology, Interleukin-10 -- biosynthesis, Interleukin-10 -- blood, Lipopolysaccharides -- pharmacology, Macrophage Activation -- drug effects, Macrophages, Peritoneal -- drug effects, Macrophages, Peritoneal -- immunology, Methotrexate -- pharmacology, Mice, Mice, Inbred BALB C, Nitric Oxide -- biosynthesis, Nitric Oxide -- blood, Tumor Necrosis Factor-alpha -- biosynthesis, Endotoxin, LPS, Methotrexate, TNF, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | No full-text files English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/57112 uri/info:pmid/9889412 uri/info:scp/0032440537 764598540 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.ocn764598540 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |