مورد إلكتروني
Design principles for nuclease-deficient CRISPR-based transcriptional regulators
| العنوان: | Design principles for nuclease-deficient CRISPR-based transcriptional regulators |
|---|---|
| المصدر: | Jensen , M K 2018 , ' Design principles for nuclease-deficient CRISPR-based transcriptional regulators ' , FEMS Yeast Research , vol. 18 , no. 4 , foy039 . |
| بيانات النشر: | 2018 |
| تفاصيل مُضافة: | Jensen, Michael K. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The engineering of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated proteins (Cas) continues to expand the toolkit available for genome editing, reprogramming gene regulation, genome visualization, and epigenetic studies of living organisms. In this review the emerging design principles on the use of nuclease-deficient CRISPR-based reprogramming of gene expression will be presented. The review will focus on the designs implemented in yeast both at the level of CRISPR proteins and gRNA, but will lend due credits to the seminal studies performed in other species where relevant. In addition to design principles, this review also highlights applications benefitting from the use of CRISPR-mediated transcriptional regulation and discuss the future directions to further expand the toolkit for nuclease-deficient reprogramming of genomes. As such this review should be of general interest for experimentalists to get familiarised with the parameters underlying the power of reprogramming genomic functions by use of nuclease-deficient CRISPR technologies. |
| مصطلحات الفهرس: | CRISPR, dCas9, Transcriptional regulation, gRNA, scRNA, dCpf1, article |
| URL: | |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | application/pdf application/pdf English |
| أرقام أخرى: | EDN oai:pure.atira.dk:publications/1ef055af-b3aa-4116-a6ee-2703924f6f50 1032629353 |
| المصدر المساهم: | TECHNICAL KNOWLEDGE CTR DENMARK From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1032629353 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |