مورد إلكتروني
Understanding the biostimulant action of vegetal-derived protein hydrolysates by high-throughput plant phenotyping and metabolomics: A case study on tomato
| العنوان: | Understanding the biostimulant action of vegetal-derived protein hydrolysates by high-throughput plant phenotyping and metabolomics: A case study on tomato |
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| المؤلفون: | Paul, Kenny, Sorrentino, Mirella, Lucini, Luigi, Rouphael, Youssef, Cardarelli, Mariateresa, Bonini, Paolo Angelo, Reynaud, Hélène, Canaguier, Renaud, Trtílek, Martin, Panzarová, Klára, Colla, Giuseppe, Lucini, Luigi (ORCID:0000-0002-5133-9464) |
| بيانات النشر: | Frontiers Media S.A. 2019 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Designing and developing new biostimulants is a crucial process which requires an accurate testing of the product effects on the morpho-physiological traits of plants and a deep understanding of the mechanism of action of selected products. Product screening approaches using omics technologies have been found to be more efficient and cost effective in finding new biostimulant substances. A screening protocol based on the use of high-throughput phenotyping platform for screening new vegetal-derived protein hydrolysates (PHs) for biostimulant activity followed by a metabolomic analysis to elucidate the mechanism of the most active PHs has been applied on tomato crop. Eight PHs (A–G, I) derived from enzymatic hydrolysis of seed proteins of Leguminosae and Brassicaceae species were foliarly sprayed twice during the trial. A non-ionic surfactant Triton X-100 at 0.1% was also added to the solutions before spraying. A control treatment foliarly sprayed with distilled water containing 0.1% Triton X-100 was also included. Untreated and PH-treated tomato plants were monitored regularly using high-throughput non-invasive imaging technologies. The phenotyping approach we used is based on automated integrative analysis of photosynthetic performance, growth analysis, and color index analysis. The digital biomass of the plants sprayed with PH was generally increased. In particular, the relative growth rate and the growth performance were significantly improved by PHs A and I, respectively, compared to the untreated control plants. Kinetic chlorophyll fluorescence imaging did not allow to differentiate the photosynthetic performance of treated and untreated plants. Finally, MS-based untargeted metabolomics analysis was performed in order to characterize the functional mechanisms of selected PHs. The treatment modulated the multi-layer regulation process that involved the ethylene precursor and polyamines and affected the ROS-mediated signaling pathways. Although further investigati |
| مصطلحات الفهرس: | Functional biostimulant characterization, Integrative image-based high-throughput phenotyping, Metabolomic, Morpho-physiological trait, Protein hydrolysate, ROS signaling, Plant Science, Settore AGR/13 - CHIMICA AGRARIA, info:eu-repo/semantics/article |
| URL: | info:eu-repo/semantics/altIdentifier/pmid/30800134 info:eu-repo/semantics/altIdentifier/wos/WOS:000458189100001 volume:10 issue:10 firstpage:1 lastpage:17 numberofpages:17 issueyear:2019 journal:FRONTIERS IN PLANT SCIENCE |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | English |
| أرقام أخرى: | SYC oai:publicatt.unicatt.it:10807/133272 10.3389/fpls.2019.00047 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85062660192 1105037276 |
| المصدر المساهم: | UNIV CATTOLICA DEL SACRO CUORE From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1105037276 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |