مورد إلكتروني
In vitro caecal fermentation of carbohydrate-rich feedstuffs in rabbits as affected by substrate pre-digestion and donors' diet
| العنوان: | In vitro caecal fermentation of carbohydrate-rich feedstuffs in rabbits as affected by substrate pre-digestion and donors' diet |
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| المؤلفون: | Ministerio de Economía y Competitividad, Comunidad de Madrid, Ministerio de Ciencia e Innovación, Ocasio-Vega, C., Abad-Guamán, R., Delgado, R., Carabaño, R., Carro, M.D., García, Javier |
| بيانات النشر: | Universitat Politècnica de València Hispana 2018-03-28 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | [EN] The influence of substrate pre-digestion and donors’ diet on in vitro caecal fermentation of different substrates in rabbits was investigated. Eight crossbreed rabbits were fed 2 experimental diets containing either low (LSF; 84.0 g/kg dry matter [DM]) or high soluble fibre (HSF; 130 g/kg DM) levels. In vitro incubations were conducted using batch cultures with soft faeces as inoculum and four fibrous or fibre-derived, low-starch and low-protein substrates: D-cellobiose (CEL), sugar beet pectin (PEC), sugar beet pulp (SBP) and wheat straw (WS). Substrates in half of the cultures were subjected to a 2-step pepsin/pancreatin in vitro digestion without filtration, and the whole residue (soluble, insoluble and added enzymes) was incubated at 39°C. Gas production was measured until 144 h, and volatile fatty acid (VFA) production at 24 h incubation was determined. Cultures without substrate (blanks) were included to correct gas production values for gas released from endogenous substrates and added enzymes. Pre-digestion had no influence on in vitro gas production kinetic of WS, and only reduced the time before gas production begins (lag time; by 31%; P=0.042) for SBP, but for both substrates the pre-digestion decreased the molar proportion of acetate (by 9%; P≤0.003) and increased those of propionate and butyrate (P≤0.014). For CEL, the pre-digestion increased the gas and total VFA production (by 30 and 114%), shortened the lag time (by 32%), and only when it was combined with LSF inoculum 38 percentage units of acetate were replaced by butyrate (P≤0.039). Treatments had a minor influence on in vitro fermentation traits of SBP pectin. The results showed that the pre-digestion process influenced the in vitro caecal fermentation in rabbits, but the effects were influenced by donors’ diet and the incubated substrate. Pre-digestion of substrate is recommended before conducting in vitro caecal fermentations. The level of soluble fibre in the donors’ diet also influenced |
| مصطلحات الفهرس: | Gas production, Processing procedure, Donors’ diet, Rabbits, Caecal fermentation, Volatile fatty acids, info:eu-repo/semantics/article |
| URL: | |
| الإتاحة: | Open access content. Open access content http://rightsstatements.org/vocab/InC/1.0 info:eu-repo/semantics/openAccess |
| ملاحظة: | TEXT English |
| أرقام أخرى: | UPV oai:riunet.upv.es:10251/100095 urn:issn:1257-5011 info:doi:10.4995/wrs.2018.7854 urn:eissn:1989-8886 1228700536 |
| المصدر المساهم: | UNIVERSITAT POLITECNICA DE VALENCIA From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1228700536 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |