مورد إلكتروني
IDENTIFIKASI AKTINOMISETES SEDIMEN AIR TAWAR MAMASA, SULAWESI BARAT DAN AKTIVITASNYA SEBAGAI ANTIBAKTERI DAN PELARUT FOSFAT
| العنوان: | IDENTIFIKASI AKTINOMISETES SEDIMEN AIR TAWAR MAMASA, SULAWESI BARAT DAN AKTIVITASNYA SEBAGAI ANTIBAKTERI DAN PELARUT FOSFAT |
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| المصدر: | Jurnal Bioteknologi & Biosains Indonesia (JBBI); Vol. 5 No. 2 (2018): December 2018; 139-148; 2548-611X; 2442-2606; 10.29122/jbbi.v5i2 |
| بيانات النشر: | Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT) 2018-12-26 |
| تفاصيل مُضافة: | Putri, Ade Lia Lisdiyanti, Puspita Kusmiati, Mia |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Identification of Actinomycetes in Freshwater Sediments from Mamasa, West Sulawesi and Their Antibacterial and Phosphate Solubilizing ActivitiesABSTRACTA large number of actinomycetes that have been isolated and screened were obtained from soil and marine samples. Consequently, the possibility of isolating novel Actinomycetes and secondary metabolites compounds strains from soil and marine samples have limited. Exploration of actinomycetes from freshwater sediment is rare. In this study, 30 isolates of Actinomycetes from freshwater sediments in Mamasa District, West Sulawesi were isolated, identified, and screened for their antibacterial and phosphate solubilizing activity. Actinomycetes were isolated by serial dilution method and were identified based on morphological and 16S rRNA gene sequence. Antibiotic activity was screened using the agar plug diffusion method, while soluble phosphate ability was observed by clear zone ratio in PKA medium. Most of the isolates belong to the genus Streptomyces (80%). Out of 30 isolates, 56.6% showed antibacterial activity and 36.6% had potential as solubilizing phosphate which belong to genus Streptomyces, Actinomadura, and Kitasatospora.Keywords: 16S rRNA, Actinomycetes, antibacterial, freshwater sediment, phosphate solubilizing ABSTRAKSebagian besar aktinomisetes yang telah diisolasi dan dilakukan penapisan metabolit sekundernya berasal dari sampel tanah dan laut. Konsekuensinya, kesempatan untuk menemukan aktinomisetes jenis baru maupun yang menghasilkan metabolit sekunder baru dari tanah dan laut semakin berkurang. Eksplorasi aktinomisetes dari lingkungan lain seperti sedimen air tawar jarang dilakukan. Pada penelitian ini, 30 isolat aktinomisetes yang diisolasi dari sedimen air tawar di Kabupaten Mamasa, Sulawesi Barat, telah diidentifikasi dan dilakukan penapisan antibakteri dan kemampuan isolat dalam melarutkan fosfat. Aktinomisetes diisolasi dengan metode pengenceran secara langsung dan selanjutnya diidentifikasi secara |
| مصطلحات الفهرس: | 16S rRNA, Aktinomisetes, sedimen air tawar, antibakteri, pelarut fosfat, Actinomycetes, info:eu-repo/semantics/article, info:eu-repo/semantics/publishedVersion, Research Articles |
| URL: | |
| الإتاحة: | Open access content. Open access content Copyright (c) 2018 Jurnal Bioteknologi & Biosains Indonesia (JBBI) |
| ملاحظة: | application/pdf application/pdf English |
| أرقام أخرى: | IDBBI oai:ejurnal.bppt.go.id:article/2953 10.29122/jbbi.v5i2.2953 1257068864 |
| المصدر المساهم: | JURNAL BIOTEKNOLOGI & BIOSAINS INDONESIA From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1257068864 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |