مورد إلكتروني
Scale-up from shake flasks to bioreactor, based on power input and Streptomyces lividans morphology, for the production of recombinant APA (45/47 kDa protein) from Mycobacterium tuberculosis.
العنوان: | Scale-up from shake flasks to bioreactor, based on power input and Streptomyces lividans morphology, for the production of recombinant APA (45/47 kDa protein) from Mycobacterium tuberculosis. |
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المؤلفون: | Universidad EAFIT. Departamento de Ciencias, Ciencias Biológicas y Bioprocesos (CIBIOP), Gamboa-Suasnavart RA, Marín-Palacio LD, Martínez-Sotelo JA, Espitia C, Servín-González L, Valdez-Cruz NA, Trujillo-Roldán MA |
المصدر: | WORLD J MICROB BIOT |
بيانات النشر: | Kluwer Academic Publishers 2021-03-23T20:08:58Z |
نوع الوثيقة: | Electronic Resource |
مستخلص: | Culture conditions in shake flasks affect filamentous Streptomyces lividans morphology, as well the productivity and O-mannosylation of recombinant Ala-Pro-rich O-glycoprotein (known as the 45/47 kDa or APA antigen) from Mycobacterium tuberculosis. In order to scale up from previous reported shake flasks to bioreactor, data from the literature on the effect of agitation on morphology of Streptomyces strains were used to obtain gassed volumetric power input values that can be used to obtain a morphology of S. lividans in bioreactor similar to the morphology previously reported in coiled/baffled shake flasks by our group. Morphology of S. lividans was successfully scaled-up, obtaining similar mycelial sizes in both scales with diameters of 0.21 ± 0.09 mm in baffled and coiled shake flasks, and 0.15 ± 0.01 mm in the bioreactor. Moreover, the specific growth rate was successfully scaled up (0.09 ± 0.02 and 0.12 ± 0.01 h(-1), for bioreactors and flasks, respectively), and the recombinant protein productivity measured by densitometry, as well. More interestingly, the quality of the recombinant glycoprotein measured as the amount of mannoses attached to the C-terminal of APA was also scaled- up; with up to five mannose residues in cultures carried out in shake flasks; and six in the bioreactor. However, final biomass concentration was not similar, indicating that although the process can be scaled-up using the power input, others factors like oxygen transfer rate, tip speed or energy dissipation/circulation function can be an influence on bacterial metabolism. |
مصطلحات الفهرس: | article, info:eu-repo/semantics/article, info:eu-repo/semantics/publishedVersion, publishedVersion, Artículo |
URL: | |
الإتاحة: | Open access content. Open access content https://v2.sherpa.ac.uk/id/publication/issn/0959-3993 |
ملاحظة: | application/pdf English |
أرقام أخرى: | CKUNE oai:repository.eafit.edu.co:10784/26818 https://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=5617 09593993 15730972 10.1007/s11274-013-1305-5 1258079702 |
المصدر المساهم: | UNIVERSIDAD EAFIT, BIBLIOTECA From OAIster®, provided by the OCLC Cooperative. |
رقم الأكسشن: | edsoai.on1258079702 |
قاعدة البيانات: | OAIster |
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