مورد إلكتروني
Mesenchymal Stem Cell-Derived Extracellular Vesicle Isolation and Their Protein Cargo Characterization
| العنوان: | Mesenchymal Stem Cell-Derived Extracellular Vesicle Isolation and Their Protein Cargo Characterization |
|---|---|
| المؤلفون: | Morente-López, Miriam, Fafián-Labora, Juan, Carrera, Mónica, Toro, Francisco J. de, Gil, Concha, Mateos, Jesús, Arufe, María C. |
| بيانات النشر: | Springer Nature 2021 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | In the present protocol, extracellular vesicles (EVs) released from a primary culture of human umbilical cord mesenchymal stem cells (MSCs) were isolated by ultracentrifugation processes, characterized by transmission electron microscopy (TEM) and measured by nanoparticle tracking analysis (NTA). Protein was extracted from EVs using RIPA buffer and then was assessed for integrity. The proteomic content of the total EV protein samples was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) after labeling by tandem mass tag (TMT). This combined approach allowed the development of an effective strategy to study the protein cargo from MSC-derived EVs |
| مصطلحات الفهرس: | Mesenchymal stem cells, Tandem mass tag, Extracellular vesicles, Transmission electron microscopy, capítulo de libro |
| URL: | Methods in Molecular Biology 2259 Sí |
| الإتاحة: | Open access content. Open access content closedAccess |
| ملاحظة: | English |
| أرقام أخرى: | CTK oai:digital.csic.es:10261/258452 Shotgun Proteomics 1: 3-12 (2021) 978-1-0716-1177-7 978-1-0716-1178-4 10.1007/978-1-0716-1178-4_1 1293838660 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1293838660 |
| قاعدة البيانات: | OAIster |
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