مورد إلكتروني
Sex-Dependent Effects of Environmental Enrichment on Spatial Memory and Brain-Derived Neurotrophic Factor (BDNF) Signaling in a Developmental 'Two-Hit' Mouse Model Combining BDNF Haploinsufficiency and Chronic Glucocorticoid Stimulation
| العنوان: | Sex-Dependent Effects of Environmental Enrichment on Spatial Memory and Brain-Derived Neurotrophic Factor (BDNF) Signaling in a Developmental 'Two-Hit' Mouse Model Combining BDNF Haploinsufficiency and Chronic Glucocorticoid Stimulation |
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| المؤلفون: | Grech, AM, Ratnayake, U, Hannan, AJ, van den Buuse, M, Hill, RA |
| بيانات النشر: | FRONTIERS MEDIA SA 2018-10-09 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Neurodevelopmental disorders are thought to be caused by a combination of adverse genetic and environmental insults. The "two-hit" hypothesis suggests that an early first "hit" primes the developing brain to be vulnerable to a second "hit" during adolescence which triggers behavioral dysfunction. We have previously modeled this scenario in mice and found that the combined effect of a genetic hapolinsuffuciency in the brain-derived neurotrophic factor (BDNF) gene (1st hit) and chronic corticosterone (CORT) treatment during adolescence (2nd hit), caused spatial memory impairments in adulthood. Environmental enrichment (EE) protocols are designed to stimulate experience-dependent plasticity and have shown therapeutic actions. This study investigated whether EE can reverse these spatial memory impairments. Wild-type (WT) and BDNF heterozygous (HET) mice were treated with corticosterone (CORT) in their drinking water (50 mg/L) from weeks 6 to 8 and exposed to EE from 7 to 9 weeks. Enriched housing included open top cages with additional toys, tunnels, housing, and platforms. Y-maze novel preference testing, to assess short-term spatial memory, was performed at 10 weeks of age. At week 16 dorsal hippocampus tissue was obtained for Western blot analysis of expression levels of BDNF, the BDNF receptor TrkB, and NMDA receptor subunits, GluNR1, 2A and 2B. As in our previous studies, spatial memory was impaired in our two-hit (BDNF HET + CORT) mice. Simultaneous EE prevented these impairments. However, EE appeared to worsen spatial memory performance in WT mice, particularly those exposed to CORT. While BDNF levels were lower in BDNF HET mice as expected, there were no further effects of CORT or EE in males but a close to significant female CORT × EE × genotype interaction which qualitatively corresponded with Y-maze performance. However, EE caused both sex- and genotype-specific effects on phosphorylated TrkB residues and GluNR expression within the dorsal hippocampus, with G |
| مصطلحات الفهرس: | Journal Article |
| URL: | NHMRC/1044777 |
| الإتاحة: | Open access content. Open access content CC BY https://creativecommons.org/licenses/by/4.0 |
| أرقام أخرى: | UMV oai:jupiter.its.unimelb.edu.au:11343/271344 Grech, A. M., Ratnayake, U., Hannan, A. J., van den Buuse, M. & Hill, R. A. (2018). Sex-Dependent Effects of Environmental Enrichment on Spatial Memory and Brain-Derived Neurotrophic Factor (BDNF) Signaling in a Developmental "Two-Hit" Mouse Model Combining BDNF Haploinsufficiency and Chronic Glucocorticoid Stimulation. FRONTIERS IN BEHAVIORAL NEUROSCIENCE, 12, https://doi.org/10.3389/fnbeh.2018.00227. 10.3389/fnbeh.2018.00227 1662-5153 1662-5153 1315713440 |
| المصدر المساهم: | UNIV OF MELBOURNE From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1315713440 |
| قاعدة البيانات: | OAIster |
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