مورد إلكتروني
Compact Microfluidic Platform with LED Light‐Actuated Valves for Enzyme‐Linked Immunosorbent Assay Automation
| العنوان: | Compact Microfluidic Platform with LED Light‐Actuated Valves for Enzyme‐Linked Immunosorbent Assay Automation |
|---|---|
| بيانات النشر: | Multidisciplinary Digital Publishing Institute 2022-05-01 |
| تفاصيل مُضافة: | European Commission Burdó‐masferrer, Mireia Díaz‐gonzález, María Sanchis, Ana Calleja, Álvaro Marco, María Pilar Fernández Sánchez, César Baldi, Antonio |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Lab‐on‐a‐chip devices incorporating valves and pumps can perform complex assays involving multiple reagents. However, the instruments used to drive these chips are complex and bulky. In this article, a new wax valve design that uses light from a light emitting diode (LED) for both opening and closing is reported. The valves and a pumping chamber are integrated in lab‐ona‐foil chips that can be fabricated at low cost using rapid prototyping techniques. A chip for the implementation of enzyme‐linked immunosorbent assays (ELISA) is designed. A porous nitrocellulose material is used for the immobilization of capture antibodies in the microchannel. A compact generic instrument with an array of 64 LEDs, a linear actuator to drive the pumping chamber, and absorbance detection for a colorimetric readout of the assay is also presented. Characterization of all the components and functionalities of the platform and the designed chip demonstrate their potential for assay automation. |
| مصطلحات الفهرس: | Wax valve, lab‐on‐a‐chip, lab‐on‐a‐foil, microfluidic ELISA, artículo |
| URL: | Biosensors Publisher's version Sí info:eu-repo/grantAgreement/EC/H2020/760921 |
| الإتاحة: | Open access content. Open access content openAccess |
| ملاحظة: | English |
| أرقام أخرى: | CTK oai:digital.csic.es:10261/270611 Biosensors 12 (5): 280 (2022) 10.3390/bios12050280 2-s2.0-85129587494 1333186262 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1333186262 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |