مورد إلكتروني
Disentangling hexaploid genetics : towards DNA-informed breeding for postharvest performance in chrysanthemum
| العنوان: | Disentangling hexaploid genetics : towards DNA-informed breeding for postharvest performance in chrysanthemum |
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| بيانات النشر: | Wageningen University 2017 |
| تفاصيل مُضافة: | van Geest, Geert |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | DNA-informed selection can strongly improve the process of plant breeding. It requires the detection of DNA polymorphisms, calculation of genetic linkage, access to reliable phenotypes and methods to detect genetic loci associated with phenotypic traits of interest. Cultivated chrysanthemum is an outcrossing hexaploid with an unknown mode of inheritance. This complicates the development of resources and methods that enable the detection of trait loci. Postharvest performance is an essential trait in chrysanthemum, but is difficult to measure. This makes it an interesting but challenging trait to phenotype and detect associated genetic loci. In this thesis I describe the development of resources and methods to enable phenotyping for postharvest performance, genetic linkage map construction and detection of quantitative trait loci in hexaploid chrysanthemum. Postharvest performance is a complicated trait because it is related to many different disorders that reduce quality. One of these disorders in chrysanthemum is disk floret degreening, which occurs after long storage. In chapter 2, we show that degreening can be prevented by feeding the flower heads with sucrose, suggesting carbohydrate starvation plays a role in the degreening process. To investigate the response to carbohydrate starvation of genotypes with different sensitivity to disk floret degreening, we investigated the metabolome of sugar-fed and carbohydrate-starved disk florets by 1H-NMR and HPAEC. We show that the metabolome is severely altered at carbohydrate starvation. In general, starvation results in an upregulation of amino acid and secondary metabolism. Underlying causes of genotypic differences explaining variation in disk floret degreening in the three investigated genotypes remained to be elucidated, but roles of regulation of respiration rate and camphor metabolism were posed as possible candidates. In chapter 3, disk floret degreening was found to be the most important postharvest disorder af |
| مصطلحات الفهرس: | chrysanthemum, dna, hexaploidy, linkage mapping, metabolomics, phenotypes, plant breeding, polymorphism, polyploidy, postharvest quality, quantitative trait loci, fenotypen, hexaploïdie, koppelingskartering, kwaliteit na de oogst, loci voor kwantitatief kenmerk, metabolomica, plantenveredeling, polymorfisme, polyploïdie, info:eu-repo/semantics/doctoralThesis, Doctoral thesis, info:eu-repo/semantics/publishedVersion |
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| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess Wageningen University & Research |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | NLWUP oai:library.wur.nl:wurpubs/526073 https://research.wur.nl/en/publications/disentangling-hexaploid-genetics-towards-dna-informed-breeding-fo 10.18174/420068 1350181994 |
| المصدر المساهم: | WUR STAFF PUBNS From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1350181994 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |