مورد إلكتروني
Tumor necrosis factor expression in human epithelial tumor cell lines.
| العنوان: | Tumor necrosis factor expression in human epithelial tumor cell lines. |
|---|---|
| المصدر: | The Journal of clinical investigation, 81 (2 |
| بيانات النشر: | 1988-02 |
| تفاصيل مُضافة: | Spriggs, D R Imamura, K Rodriguez, C. Sariban, Eric Kufe, D W |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Tumor necrosis factor (TNF) is a monokine with in vitro cytotoxicity for some but not all tumor cells. The basis for sensitivity and resistance to the antitumor effects of this agent remains unclear. The present studies have monitored the effects of TNF on 14 epithelial tumor cell lines. Eleven of these cell lines were resistant to the growth inhibitory effects of TNF (50% inhibitory concentration greater than 1,000 U/ml). 12 of the 14 tumor cell lines has detectable levels of high affinity cell surface TNF binding sites, thus suggesting that resistance was not often due to the absence of cell surface TNF receptors. Northern blot analysis demonstrated that three of the eleven resistant cell lines expressed detectable levels of TNF mRNA. Furthermore, both sensitive and resistant epithelial tumor cells had the capacity to express TNF transcripts in the presence of the protein synthesis inhibitor, cycloheximide. Finally, the presence of TNF expression at the RNA level is shown to be associated with the production of a TNF-like protein in the resistant Ov-D ovarian carcinoma cells. These findings suggest that certain human epithelial tumor cell lines inherently resistant to TNF also express this cytokine. Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Animals, Cell Division -- drug effects, Cycloheximide -- pharmacology, Epithelium -- physiology, Gene Expression Regulation -- drug effects, Humans, Mice, RNA, Messenger -- genetics, Receptors, Cell Surface -- metabolism, Receptors, Tumor Necrosis Factor, Tumor Cells, Cultured -- physiology, Tumor Necrosis Factor-alpha -- genetics, Tumor Necrosis Factor-alpha -- pharmacology, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content 1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | 1 full-text file(s): application/pdf English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/73189 uri/info:doi/10.1172/JCI113341 uri/info:pmid/2828427 uri/info:pmcid/PMC329591 1363679105 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1363679105 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |