مورد إلكتروني
Characterization of subunits of the RNA polymerase I complex in Trypanosoma brucei.
| العنوان: | Characterization of subunits of the RNA polymerase I complex in Trypanosoma brucei. |
|---|---|
| المؤلفون: | Walgraffe, David, Devaux, Sara, Lecordier, Laurence, Dierick, Jean-Francois, Dieu, M., Van Den Abbeele, J, Pays, Etienne, Vanhamme, Luc |
| المصدر: | Molecular and biochemical parasitology, 139 (2 |
| بيانات النشر: | 2005-02 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The Trypanosoma brucei homologue of the RNA polymerase I (RNA Pol I) subunit Rpa12p of Saccharomyces cerevisiae was cloned and characterized. This protein did not appear to be essential for growth in either bloodstream or procyclic forms of the parasite. Trypanosomes expressing a C-terminal tagged version of TbRPA12 were generated in order to purify RNA Pol I from both developmental stages. Tandem affinity purification (TAP) revealed a number of proteins associating with TbRPA12, some of which appeared to be stage-specific. Mass spectrometry allowed the identification of four subunits in addition to TbRPA12, namely TbRPA1, TbRPA2, TbRPC40 and one isoform of TbRPB5 (Tb1RPB5), as well as an unknown 30kDa protein and histones H2A and H3. Whereas these studies demonstrated that TbRPA1 was phosphorylated, no evidence for phosphorylation of TbRPA2 was found. Journal Article info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Amino Acid Sequence, Animals, Antigenic Variation, Gene Expression Regulation, Developmental, Life Cycle Stages, Molecular Sequence Data, Protein Subunits -- genetics, Protein Subunits -- metabolism, RNA Polymerase I -- chemistry, RNA Polymerase I -- genetics, RNA Polymerase I -- metabolism, Recombinant Proteins, Saccharomyces cerevisiae -- enzymology, Saccharomyces cerevisiae -- genetics, Saccharomyces cerevisiae Proteins -- chemistry, Saccharomyces cerevisiae Proteins -- genetics, Saccharomyces cerevisiae Proteins -- metabolism, Sequence Alignment, Trypanosoma brucei brucei -- enzymology, Trypanosoma brucei brucei -- genetics, Trypanosoma brucei brucei -- growth & development, Antigenic variation, Polycistronic transcription, Trypanosoma brucei, VSG expression sites, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content 1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | 1 full-text file(s): application/pdf English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/51549 uri/info:doi/10.1016/j.molbiopara.2004.11.014 uri/info:pii/S0166-6851(04)00318-4 uri/info:pmid/15664659 uri/info:scp/12344305492 1363712508 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1363712508 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |