مورد إلكتروني
Biofunctionalization of 3D printed collagen with bevacizumab-loaded microparticles targeting pathological angiogenesis
| العنوان: | Biofunctionalization of 3D printed collagen with bevacizumab-loaded microparticles targeting pathological angiogenesis |
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| المؤلفون: | Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica, Abbadessa, Anna, Núñez Bernal, Paulina, Buttitta, Giorgio, Ronca, Alfredo, D'Amora, Ugo, Zihlmann, Carla, Stiefel, Niklaus, Ambrosio, Luigi, Malda, Jos, Levato, Riccardo, Crecente Campo, José, Alonso Fernández, María José |
| بيانات النشر: | Elsevier 2023 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Pathological angiogenesis is a crucial attribute of several chronic diseases such as cancer, age-related macular degeneration, and osteoarthritis (OA). In the case of OA, pathological angiogenesis mediated by the vascular endothelial growth factor (VEGF), among other factors, contributes to cartilage degeneration and to implants rejection. In line with this, the use of the anti-VEGF bevacizumab (BVZ) has been shown to prevent OA progression and support cartilage regeneration. The aim of this work was to functionalize a medical grade collagen with poly (lactic-co-glycolic acid) (PLGA) microparticles containing BVZ via three-dimensional (3D) printing to target pathological angiogenesis. First, the effect of several formulation parameters on the encapsulation and release of BVZ from PLGA microparticles was studied. Then, the anti-angiogenic activity of released BVZ was tested in a 3D cell model. The 3D printability of the microparticle-loaded collagen ink was tested by evaluating the shape fidelity of 3D printed structures. Results showed that the release and the encapsulation efficiency of BVZ could be tuned as a function of several formulation parameters. In addition, the released BVZ was observed to reduce vascularization by human umbilical vein endothelial cells. Finally, the collagen ink with embedded BVZ microparticles was successfully printed, leading to shape-stable meniscus-, nose- and auricle-like structures. Taken altogether, we defined the conditions for the successful combination of BVZ-loaded microparticles with the 3D printing of a medical grade collagen to target pathological angiogenesis |
| مصطلحات الفهرس: | journal article |
| URL: | info:eu-repo/grantAgreement/EC/H2020-814444 (MEFISTO) info:eu-repo/grantAgreement/EC/H2020-949806 (VOLUME-BIO) |
| الإتاحة: | Open access content. Open access content http://creativecommons.org/licenses/by-nc-nd/4.0 open access © 2023 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/) Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
| ملاحظة: | English |
| أرقام أخرى: | ESUSC oai:minerva.usc.es:10347/30956 Journal of Controlled Release 360 (2023) 747-758 10.1016/j.jconrel.2023.07.017 0168-3659 1401001865 |
| المصدر المساهم: | UNIVERSIDADE DE SANTIAGO DE COMPOSTELA From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1401001865 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |