مورد إلكتروني
Disposable electrochemical biosensors for Brettanomyces bruxellensis and total yeast content in wine based on core-shell magnetic nanoparticles
| العنوان: | Disposable electrochemical biosensors for Brettanomyces bruxellensis and total yeast content in wine based on core-shell magnetic nanoparticles |
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| المؤلفون: | Villalonga, María L., Borisova, Boryana, Arenas, Christian B., Villalonga, Anabel, Arévalo-Villena, María, Sánchez Sánchez, Alfredo, Pingarrón Carrazón, José Manuel, Briones-Pérez, Ana, Villalonga Santana, Reynaldo |
| بيانات النشر: | Elsevier B.V. 2024-01-12T09:26:52Z 2024-01-12T09:26:52Z 2018-09-24 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Two different disposable amperometric biosensors for the rapid detection and quantification of Brettanomyces bruxellensis (Brett) and total yeast content in wine were constructed. The biosensing approach implied the use of magnetic capturing elements based on core-shell Fe3O4@SiO2 superparamagnetic nanoparticles (NanoCaptors) functionalized with specific bioreceptors: Concanavalin A (Con A) for total yeast analysis, and anti-Brett polyclonal antibodies for Brett. A Con A-peroxidase conjugate and carbon screen printed electrodes were used as signaling element and sensing interfaces, respectively. The biosensor based on antibody-modified magnetic nanoparticles allowed the amperometric detection of B. bruxellensis in buffer solution and red wine samples in the range of 10 - 106 CFU/mL, with low detection limits of 6 CFU/mL and 8 CFU/mL, respectively. In addition, the use of Con A-linked magnetic nanoparticles as capturing elements allowed determination of total yeast content in buffer solution and red wine in the range of 10 - 106 CFU/mL, with detection limit of 5 CFU/mL. These electrochemical biosensors also exhibited high reproducibility, selectivity and storage stability. Ministerio de Economia y ́Competitividad Junta de Comunidades de Castilla La Mancha Comunidad de Madrid Depto. de Química Analítica Fac. de Ciencias Químicas TRUE pub |
| مصطلحات الفهرس: | 543, Brettanomyces bruxellensis, Saccharomyces cerevisiae, Biosensor, Yeast, Red wine, Screen printed electrodes, Ciencias, 23 Química, journal article, VoR |
| URL: | (MINECO Projects CTQ2014-58989-P, CTQ2015- 71936-REDT and CTQ2017-87954-P) (JCCM Project POII-2014-011-A) NANOAVANSENS (Project S2013/ MIT-3029) |
| الإتاحة: | Open access content. Open access content Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0 open access |
| ملاحظة: | application/pdf 0925-4005 English |
| أرقام أخرى: | ESRCM oai:docta.ucm.es:20.500.14352/92685 Villalonga, M. L., Borisova, B., Arenas, C. B., Villalonga, A., Arévalo-Villena, M., Sánchez, A., Pingarrón, J. M., Briones-Pérez. A., Villalonga, R.* (2019). Disposable electrochemical biosensors for Brettanomyces bruxellensis and total yeast content in wine based on core-shell magnetic nanoparticles. Sensors and Actuators B: Chemical, 279, 15-21. 0925-4005 10.1016/j.snb.2018.09.092 1429625823 |
| المصدر المساهم: | REPOSITORIO E-PRINTS UNIVERSIDAD COMPLU From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1429625823 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |