دورية أكاديمية
An immuno-PF2D-MS/MS proteomic approach for bacterial antigenic characterization: To Bacillus and beyond
| العنوان: | An immuno-PF2D-MS/MS proteomic approach for bacterial antigenic characterization: To Bacillus and beyond |
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| المؤلفون: | Ruelle, Virginie, Falisse-Poirier, Nandini, Elmoualij, Benaïssa, Zorzi, Danièle, Pierard, Olivier, Heinen, Ernst, De Pauw, Edwin, Zorzi, Willy |
| المساهمون: | Giga-Systems Biology and Chemical Biology - ULiège, CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège |
| المصدر: | Journal of Proteome Research, 6 (6), 2168-2175 (2007-06) |
| بيانات النشر: | Amer Chemical Soc, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Bacillus subtilis, flagellin, in-gel digest, mass spectrometry, PF2D, proteomics, Life sciences, Biochemistry, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/ or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled to immunological techniques and mass spectrometry ( i-PF2D-MS/MS). To evaluate this strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial model. IMALDI |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article |
| اللغة: | English |
| Relation: | urn:issn:1535-3893; urn:issn:1535-3907 |
| DOI: | 10.1021/pr060661g |
| URL الوصول: | https://orbi.uliege.be/handle/2268/24919 |
| رقم الأكسشن: | edsorb.24919 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1021/pr060661g |
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