دورية أكاديمية
Genetic mapping of folate QTLs using a segregated population in maize.
| العنوان: | Genetic mapping of folate QTLs using a segregated population in maize. |
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| المؤلفون: | Guo, Wenzhu, Lian, Tong, Wang, Baobao, Guan, Jiantao, Yuan, Dong, Wang, Huan, Safiul Azam, Fardous Mohammad, Wan, Xing, Wang, Weixuan, Liang, Qiuju, Wang, Haiyang, Tu, Jinxing, Zhang, Chunyi, Jiang, Ling |
| المصدر: | Journal of Integrative Plant Biology, 61 (6), 675-690 (2019-06) |
| بيانات النشر: | Blackwell Publishing Ltd, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Tetrahydrofolates, 5,6,7,8-tetrahydrofolic acid, Folic Acid, Chromosome Segregation/genetics, Chromosomes, Plant/genetics, Crosses, Genetic, Folic Acid/metabolism, Genetic Association Studies, Genetic Linkage, Phenotype, Quantitative Trait Loci/genetics, Recombination, Genetic/genetics, Tetrahydrofolates/metabolism, Whole Exome Sequencing, Zea mays/genetics, Chromosome Mapping, Chromosome Segregation, Chromosomes, Plant, Quantitative Trait Loci, Recombination, Genetic, Zea mays, Biochemistry, Biochemistry, Genetics and Molecular Biology (all), Plant Science, General Biochemistry, Genetics and Molecular Biology, Life sciences, Genetics & genetic processes, Sciences du vivant, Génétique & processus génétiques |
| الوصف: | As essential B vitamin for humans, folates accumulation in edible parts of crops, such as maize kernels, is of great importance for human health. But its breeding is always limited by the prohibitive cost of folate profiling. The molecular breeding is a more executable and efficient way for folate fortification, but is limited by the molecular knowledge of folate regulation. Here we report the genetic mapping of folate quantitative trait loci (QTLs) using a segregated population crossed by two maize lines, one high in folate (GEMS31) and the other low in folate (DAN3130). Two folate QTLs on chromosome 5 were obtained by the combination of F2 whole-exome sequencing and F3 kernel-folate profiling. These two QTLs had been confirmed by bulk segregant analysis using F6 pooled DNA and F7 kernel-folate profiling, and were overlapped with QTLs identified by another segregated population. These two QTLs contributed 41.6% of phenotypic variation of 5-formyltetrahydrofolate, the most abundant storage form among folate derivatives in dry maize grains, in the GEMS31×DAN3130 population. Their fine mapping and functional analysis will reveal details of folate metabolism, and provide a basis for marker-assisted breeding aimed at the enrichment of folates in maize kernels. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article peer reviewed |
| اللغة: | English |
| Relation: | https://onlinelibrary.wiley.com/doi/pdf/10.1111/jipb.12811; urn:issn:1672-9072; urn:issn:1744-7909 |
| DOI: | 10.1111/jipb.12811 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/291340 |
| حقوق: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| رقم الأكسشن: | edsorb.291340 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1111/jipb.12811 |
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