دورية أكاديمية
Effect of dexamethasone on gene expression of cannabinoid receptor type 1 and adenosine monophosphate-activated protein kinase in the hypothalamus of broilers (Gallus domesticus).
| العنوان: | Effect of dexamethasone on gene expression of cannabinoid receptor type 1 and adenosine monophosphate-activated protein kinase in the hypothalamus of broilers (Gallus domesticus). |
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| المؤلفون: | Kong, Linglian, Xiao, Chuanpi, Lin, Hai, Buyse, Johan, Li, Xianlei, Song, Zhigang |
| المصدر: | Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology, 260, 111018 (2021-10) |
| بيانات النشر: | Elsevier BV, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adenosine monophosphate-activated protein kinase, Broilers, Cannabinoid receptor type 1, Dexamethasone, Stress, Cnr1 protein, rat, Glucocorticoids, RNA, Messenger, Receptor, Cannabinoid, CB1, Receptors, Glucocorticoid, AMP-Activated Protein Kinases, Animals, Chickens, Dexamethasone/pharmacology, Glucocorticoids/metabolism, Hypothalamus/metabolism, Male, RNA, Messenger/metabolism, Receptor, Cannabinoid, CB1/metabolism, Receptors, Glucocorticoid/genetics, Signal Transduction, Gene Expression Profiling, Gene Expression Regulation, Molecular Biology, Physiology, Biochemistry, Life sciences, Anatomy (cytology, histology, embryology...) & physiology, Sciences du vivant, Anatomie (cytologie, histologie, embryologie...) & physiologie |
| الوصف: | Hypothalamic neural circuits play a critical role in integrating peripheral signals and conveying information about energy and nutrient status. We detected cannabinoid receptor type 1 (CB1) distribution in the hypothalamus, liver, duodenum, jejunum, and ileum among 7- and 35-day-old broilers. The effects of dexamethasone (DEX) on CB1 gene expression were evaluated by in vitro and in vivo experiments on glucocorticoid receptor (GR) and adenosine monophosphate-activated protein kinase (AMPK) in the hypothalamus of broilers. In vitro, hypothalamic cells from 17-day-old broiler embryos were incubated with either 0.1% dimethyl sulfoxide or DEX (100 nmol/mL) for 1 h. In the in vivo study, 28-day-old broilers were injected with DEX for 24 h or 72 h. Results showed that CB1 was mainly expressed in the hypothalamus, and 72 h DEX treatment increased the expression. One-day treatment of broilers with DEX did not change the hypothalamic CB1 gene expression. Moreover, DEX treatment for 24 h and 72 h increased the mRNA level of hypothalamic AMPKα2 and GR. However, no differences were observed on the gene expression of CB1, GR, and AMPKα2 in hypothalamic cells with DEX-treated for 1 h. In conclusion, CB1 is mainly expressed in the hypothalamus of broilers; 72-h DEX exposure can regulate the CB1 system and AMPK signaling pathway of the broiler hypothalamus. |
| نوع الوثيقة: | journal article http://purl.org/coar/resource_type/c_6501 article peer reviewed |
| اللغة: | English |
| Relation: | https://api.elsevier.com/content/article/PII:S1095643321001264?httpAccept=text/xml; urn:issn:1095-6433; urn:issn:1531-4332 |
| DOI: | 10.1016/j.cbpa.2021.111018 |
| URL الوصول: | https://orbi.uliege.be/handle/2268/316295 |
| حقوق: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| رقم الأكسشن: | edsorb.316295 |
| قاعدة البيانات: | ORBi |
| DOI: | 10.1016/j.cbpa.2021.111018 |
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