This paper presents geophysical and geochemical evidence for the possible existence of cold, subducted lithosphere beneath the Saint Paul Fracture Zone of the Equatorial Atlantic Ocean. The ocean floor along the fracture zone is characterized by a high percentage of abyssal peridotites. The abyssal peridotites were emplaced by tectonic uplift of the oceanic lithosphere. The top of the ridge is exposed at Saint Peter and Saint Paul islets. The Os isotopic composition of these abyssal peridotites indicate the presence of old depleted mantle material in this region with Re-depletion model ages (T RD) from 0.32 to 1.1Ga. Melt inclusions in plagioclase phenocrysts of the MORB close to this location have boninitic composition, suggesting that some basalts originated from low-degree mantle melting. The global tomography models show fast seismic velocities in the upper and lower mantle of the Equatorial Atlantic Ocean, consistent with the presence of cold subducted lithosphere. The range of Re-depletion model ages are consistent with paleo-reconstructions of plate motion, suggesting that the fossil subducted slab was formed during the closure of both the Iapetus and the Rheic oceans.