The application of biotechnology in animal reproduction has enabled the production of young forms in both quantity and quality. Increasing the number of viable gametes produced by reproducers, among other factors, through an ideal diet, can ensure higher production. Therefore, the aim of this study was to determine the influence of three diets on the sperm survival of Macrobrachium acanthurus. To this end, 24 M. acanthurus males were used, distributed randomly and equally among treatments. Their diets were composed of 100% fresh food (fish and squid muscle - 14% protein), 100% dry feed (commercial feed - 50% protein) and a mixture of these diets containing 30% protein. Spermatophores were extracted through electrical stimulation every 15 days, and the controls consisted of spermatophores obtained directly from nature. No significant difference between diets was observed comparing shrimp and spermatophore weights. The 100% fresh diet provided the best sperm survival performance.
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