In vitro Blood-Brain barrier model based on recombinant spider silk protein nanomembranes for evaluation of transcytosis capability of biomolecules
| العنوان: | In vitro Blood-Brain barrier model based on recombinant spider silk protein nanomembranes for evaluation of transcytosis capability of biomolecules |
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| المؤلفون: | Hjelm, Linnea Charlotta, 1993, Hedhammar, My, Professor, 1975, Löfblom, John |
| المصدر: | Biochemical and Biophysical Research Communications - BBRC. 669:77-84 |
| مصطلحات موضوعية: | Blood-brain barrier, Transwell assay, Transferrin Receptor-1, Recombinant spider silk, Receptor-mediated transcytosis |
| الوصف: | The blood-brain barrier (BBB) limits the uptake of central nervous system (CNS)-targeting drugs into the brain. Engineering molecular shuttles for active transportation across the barrier has thus potential for improving the efficacy of such drugs. In vitro assessment of potential transcytosis capability for engi-neered shuttle proteins facilitates ranking and the selection of promising candidates during develop-ment. Herein, the development of an assay based on brain endothelial cells cultured on permeable recombinant silk nanomembranes for screening of transcytosis capability of biomolecules is described. The silk nanomembranes supported growth of brain endothelial cells to form confluent monolayers with relevant cell morphology, and induced expression of tight-junction proteins. Evaluation of the assay using an established BBB shuttle antibody showed transcytosis over the membranes with an apparent permeability that significantly differed from the isotype control antibody. |
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| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-331196 https://doi.org/10.1016/j.bbrc.2023.05.093 |
| قاعدة البيانات: | SwePub |
| تدمد: | 0006291X 10902104 |
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| DOI: | 10.1016/j.bbrc.2023.05.093 |