Interleukin-13 Treatment of Living Lung Tissue Model Alters the Metabolome and Proteome: A Nano-DESI MS Metabolomics and Shotgun Proteomics Study
| العنوان: | Interleukin-13 Treatment of Living Lung Tissue Model Alters the Metabolome and Proteome: A Nano-DESI MS Metabolomics and Shotgun Proteomics Study |
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| المؤلفون: | Tóth, Gábor, Golubova, Anastasia, 1996, Falk, Alexander, Research engineer, 1986, Bergström Lind, Sara, 1977, Nicholas, Mark, Lanekoff, Ingela, Prof., 1975 |
| المصدر: | International Journal of Molecular Sciences. 25(9) |
| مصطلحات موضوعية: | asthma, interleukin-13, nano-DESI, proteomics, metabolomics, multi-omics, air-liquid interface, amino acid |
| الوصف: | Asthma is a chronic respiratory disease with one of the largest numbers of cases in the world; thus, constant investigation and technical development are needed to unravel the underlying biochemical mechanisms. In this study, we aimed to develop a nano-DESI MS method for the in vivo characterization of the cellular metabolome. Using air-liquid interface (ALI) cell layers, we studied the role of Interleukin-13 (IL-13) on differentiated lung epithelial cells acting as a lung tissue model. We demonstrate the feasibility of nano-DESI MS for the in vivo monitoring of basal-apical molecular transport, and the subsequent endogenous metabolic response, for the first time. Conserving the integrity of the ALI lung-cell layer enabled us to perform temporally resolved metabolomic characterization followed by "bottom-up" proteomics on the same population of cells. Metabolic remodeling was observed upon histamine and corticosteroid treatment of the IL-13-exposed lung cell monolayers, in correlation with alterations in the proteomic profile. This proof of principle study demonstrates the utility of in vivo nano-DESI MS for characterizing ALI tissue layers, and the new markers identified in our study provide a good starting point for future, larger-scale studies. |
| وصف الملف: | electronic |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-528692 https://doi.org/10.3390/ijms25095034 https://uu.diva-portal.org/smash/get/diva2:1863467/FULLTEXT01.pdf |
| قاعدة البيانات: | SwePub |
| تدمد: | 20134231 |
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| DOI: | 10.3390/ijms25095034 |