Effects of interleukin-6 and interleukin-1 beta on expression of growth differentiation factor-5 and Wnt signaling pathway genes in equine chondrocytes
| العنوان: | Effects of interleukin-6 and interleukin-1 beta on expression of growth differentiation factor-5 and Wnt signaling pathway genes in equine chondrocytes |
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| المؤلفون: | Svala, E., Thorfve, Anna, 1982, Ley, C., Barreto Henriksson, Helena, Synnergren, J. M., Lindahl, Anders, 1954, Ekman, S., Skioldehrand, E. S. R. |
| المصدر: | American Journal of Veterinary Research. 75(2):132-140 |
| مصطلحات موضوعية: | Clinical Medicine, Klinisk medicin, HUMAN ARTICULAR CHONDROCYTES, BONE MORPHOGENETIC PROTEINS, TUMOR-NECROSIS-FACTOR, BETA-CATENIN, SYNOVIAL-FLUID, WNT/BETA-CATENIN, IN-VITRO, BINDING PROTEIN, JOINT FORMATION, CARTILAGE, OL RR, VETERINARY CLINICS OF NORTH AMERICA-EQUINE PRACTICE, V6, P1 |
| الوصف: | Objective-To determine the effects of interleukin (IL)-6 and IL-1 beta stimulation on expression of growth differentiation factor (GDF)-5 and Wnt signaling pathway genes in equine chondrocytes. Sample-Macroscopically normal articular cartilage samples from 6 horses and osteochondral fragments (OCFs) from 3 horses. Procedures-Chondrocyte pellets were prepared and cultured without stimulation or following stimulation with IL-6 or IL-1 beta for 1, 2, 12, and 48 hours; expression of GDF-5 was determined with a quantitative real-time PCR assay. Expression of genes in various signaling pathways was determined with microarrays for pellets stimulated for 1 and 2 hours. Immunohistochemical analysis was used to detect GDF-5, glycogen synthase kinase 3 beta (GSK-3 beta), and beta-catenin proteins in macroscopically normal cartilage samples and OCFs. Results-Chondrocytes stimulated with IL-6 had significantly higher GDF-5 expression within 2 hours versus unstimulated chondrocytes. Microarray analysis of Wnt signaling pathway genes indicated expression of GSK-3 beta and coiled-coil domain containing 88C increased after 1 hour and expression of beta-catenin decreased after 2 hours of IL-6 stimulation. Results of immunohistochemical detection of proteins were similar to microarray analysis results. Chondrocytes in macroscopically normal articular cartilage and OCFs had immunostaining for GDF-5. Conclusion and Clinical Relevance-Results indicated IL-6 stimulation decreased chondrocyte expression of the canonical Wnt signaling pathway transactivator beta-catenin, induced expression of inhibitors of the Wnt pathway, and increased expression of GDF-5. This suggested IL-6 may inhibit the Wnt signaling pathway with subsequent upregulation of GDF-5 expression. Anabolic extracellular matrix metabolism in OCFs may be attributable to GDF-5 expression. This information could be useful for development of cartilage repair methods. |
| URL الوصول: | https://gup.ub.gu.se/publication/194990 |
| قاعدة البيانات: | SwePub |
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