دورية أكاديمية
Proposal of a Laboratory Course Dedicated to the Generation of Protein Molecular Weight Standards for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
العنوان: | Proposal of a Laboratory Course Dedicated to the Generation of Protein Molecular Weight Standards for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis |
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اللغة: | English |
المؤلفون: | Bubis, José (ORCID |
المصدر: | Biochemistry and Molecular Biology Education. May-Jun 2021 49(3):353-360. |
الإتاحة: | Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us |
Peer Reviewed: | Y |
Page Count: | 8 |
تاريخ النشر: | 2021 |
نوع الوثيقة: | Journal Articles Reports - Descriptive |
Descriptors: | Molecular Biology, Science Laboratories, Molecular Structure, Scientific Concepts, Chemistry |
DOI: | 10.1002/bmb.21476 |
تدمد: | 1470-8175 |
مستخلص: | Protein molecular weight standards are routinely employed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the apparent sizes of unknown proteins within a sample. For training students, a laboratory course based on the production of marker proteins is proposed. Following fractionation by column chromatography, a series of purified proteins and mixtures of proteins were combined to generate various sets of unstained, prestained and fluorescently labeled molecular weight ladders for SDS-PAGE. The produced material can be used in successive laboratory practices as an alternative to commercial protein markers. |
Abstractor: | As Provided |
Entry Date: | 2021 |
رقم الأكسشن: | EJ1295158 |
قاعدة البيانات: | ERIC |
تدمد: | 1470-8175 |
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DOI: | 10.1002/bmb.21476 |