دورية أكاديمية
Proposal of a Laboratory Course Dedicated to the Generation of Protein Molecular Weight Standards for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
| العنوان: | Proposal of a Laboratory Course Dedicated to the Generation of Protein Molecular Weight Standards for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis |
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| اللغة: | English |
| المؤلفون: | Bubis, José (ORCID |
| المصدر: | Biochemistry and Molecular Biology Education. May-Jun 2021 49(3):353-360. |
| الإتاحة: | Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us |
| Peer Reviewed: | Y |
| Page Count: | 8 |
| تاريخ النشر: | 2021 |
| نوع الوثيقة: | Journal Articles Reports - Descriptive |
| Descriptors: | Molecular Biology, Science Laboratories, Molecular Structure, Scientific Concepts, Chemistry |
| DOI: | 10.1002/bmb.21476 |
| تدمد: | 1470-8175 |
| مستخلص: | Protein molecular weight standards are routinely employed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the apparent sizes of unknown proteins within a sample. For training students, a laboratory course based on the production of marker proteins is proposed. Following fractionation by column chromatography, a series of purified proteins and mixtures of proteins were combined to generate various sets of unstained, prestained and fluorescently labeled molecular weight ladders for SDS-PAGE. The produced material can be used in successive laboratory practices as an alternative to commercial protein markers. |
| Abstractor: | As Provided |
| Entry Date: | 2021 |
| رقم الأكسشن: | EJ1295158 |
| قاعدة البيانات: | ERIC |
Full Text Finder | تدمد: | 1470-8175 |
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| DOI: | 10.1002/bmb.21476 |