دورية أكاديمية
Characterization of Proteinous Coagulant in 'Moringa' Tree Seeds for Water Purification: Stepwise Laboratory Exercise for High-School Students
| العنوان: | Characterization of Proteinous Coagulant in 'Moringa' Tree Seeds for Water Purification: Stepwise Laboratory Exercise for High-School Students |
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| اللغة: | English |
| المؤلفون: | Oyama, Hiroshi (ORCID |
| المصدر: | Biochemistry and Molecular Biology Education. Sep-Oct 2023 51(5):588-599. |
| الإتاحة: | Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us |
| Peer Reviewed: | Y |
| Page Count: | 12 |
| تاريخ النشر: | 2023 |
| نوع الوثيقة: | Journal Articles Reports - Research |
| Education Level: | High Schools Secondary Education |
| Descriptors: | Science Education, Science Laboratories, High School Students, Hands on Science, Pollution, Water, Intervention, Biochemistry, Scientific Concepts, Water Quality, Secondary School Science |
| DOI: | 10.1002/bmb.21763 |
| تدمد: | 1470-8175 1539-3429 |
| مستخلص: | Coagulation is an important process in the context of water purification; and the seed protein of the moringa tree ("Moringa oleifera") is a remarkably effective coagulant. The laboratory course described here is designed to provide high-school students with a stepwise, hands-on experience in investigating the protein-rich coagulant found in "Moringa" seeds. First, the seed powder was applied to model polluted water containing fine clay, food dyes, copper sulfate, and bacteria. This treatment changed the polluted water into clear water via coagulation; all students were convinced that the coagulation-inducing agent was a thermostable cationic protein. Finally, basic biochemical techniques (e.g., chromatographic separation and electrophoresis) were used to show that the target coagulant is a dimeric protein composed of 6.5 and 4.5 kDa subunits. Overall, this made it possible for the students to gain a deeper understanding (more comprehensive than the information taught in formal classes) of protein structure and its real-world implications. This stepwise exercise can be applied to research-based learning programs in high school, as it is an effective learning tool. |
| Abstractor: | As Provided |
| Entry Date: | 2023 |
| رقم الأكسشن: | EJ1395346 |
| قاعدة البيانات: | ERIC |
Full Text Finder | تدمد: | 1470-8175 1539-3429 |
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| DOI: | 10.1002/bmb.21763 |